Consumption of polyphenol concentrate with dietary fructo-oligosaccharides enhances cecal metabolism of quercetin glycosides in rats.
ABSTRACT We verified the hypothesis that the consumption of polyphenol concentrate (PC), rich in quercetin and its glycosides (36 g/100 g), in association with different dietary fiber matrices, that is, an easily fermentable fructo-oligosaccharides (FOS) or non-fermentable cellulose (CEL), causes a disparate adaptive response of the cecal microbial activity in rats. This in turn facilitates further utilization of biologically active polyphenolic compounds, which are not, as usual, digested in the foregut.
Four-week experimental feeding of male Wistar rats consisted of diets containing 5% FOS or CEL, as a source of dietary fiber, with or without 0.3% addition of PC.
Positive changes in rat cecum were observed resulting from the ingestion of an FOS-containing diet, such as decreased pH and increased the production of short-chain fatty acids in the digesta, compared with a CEL-containing diet. The addition of PC to the FOS diet did not eliminate the positive effects of the latter, except for a slight increase in cecal pH and a decrease in microbial glycolytic activity. However, a simultaneous increase in the cecal butyrate pool was also observed. An adaptation process of the microflora enzymatic system to dieting with PC and FOS was proven in further enhanced susceptibility of rutin (quercetin 3-O-glucorhamnoside), hyperoside (quercetin 3-O-galactoside), and quercitrin (quercetin 3-O-rhamnoside) to hydrolysis by the cecal digesta solution.
Especially when consumed together, PC and FOS are important dietary factors affecting the susceptibility of quercetin glycosides to microbial metabolism in the cecum. The intensification of the hydrolysis of quercetin glycosides by dietary treatments leads also to the increased metabolism of quercetin itself.
Article: Identification of microbial metabolites derived from in vitro fecal fermentation of different polyphenolic food sources.[show abstract] [hide abstract]
ABSTRACT: The biological effects of dietary polyphenols are linked to their bioavailability and catabolism in humans. The colon, with its symbiotic microbiota, is an active site where complex polyphenolic compounds are possibly modified to smaller and more absorbable molecules. The aim of this study was to identify the major metabolites derived from microbial colonic fermentation of some common polyphenol-rich foods. An in vitro fecal fermentation model was applied to 16 polyphenol-rich foods and polyphenolic precursors. Phenolic metabolites were identified by high-performance liquid chromatography coupled with tandem mass spectrometric detection. Twenty-four phenolic fermentation metabolites were characterized. Some metabolites were common to several polyphenol-rich foods, whereas others were characteristic of specific sources. The metabolites identified in vitro likely are generated in the human colon after consumption of polyphenol-rich foods. Their occurrence in plasma and/or urine should be considered when evaluating the bioavailability of polyphenols from specific food groups in humans and in the definition of markers of exposure to specific foods or food groups in epidemiologic studies. However, the search for these and other microbial metabolites after a feeding study in vivo should consider their possible further conjugation at the level of the liver.Nutrition 02/2012; 28(2):197-203. · 3.03 Impact Factor