Live optical projection tomography

Centre for Genomic Regulation
Organogenesis (Impact Factor: 2.6). 10/2009; 5(4):211-6. DOI: 10.4161/org.5.4.10426
Source: PubMed

ABSTRACT Optical projection tomography (OPT) is a technology ideally suited for imaging embryonic organs. We emphasize here recent successes in translating this potential into the field of live imaging. Live OPT (also known as 4D OPT, or time-lapse OPT) is already in position to accumulate good quantitative data on the developmental dynamics of organogenesis, a prerequisite for building realistic computer models and tackling new biological problems. Yet, live OPT is being further developed by merging state-of-the-art mouse embryo culture with the OPT system. We discuss the technological challenges that this entails and the prospects for expansion of this molecular imaging technique into a wider range of applications.

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Available from: jean-francois Colas, May 10, 2014
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    • "As a consequence, the overall resolution and contrast in the OPT reconstructions are compromised. Moreover, SPIM is considered an ideal technique to image development in vivo, whereas tomographic imaging is commonly believed suitable only for fixed and chemically cleared samples and, indeed, only a few in vivo applications have been reported (Colas and Sharpe, 2009; Bassi et al., 2011; McGinty et al., 2011). We decided to look for alternative solutions that would achieve tomographic reconstructions of living specimens in a highresolution , high-NA SPIM setup without any modifications to the hardware. "
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