Success stories in molecular farming—A brief overview

Plant Biotechnology Journal (Impact Factor: 5.75). 06/2010; 8(5):525-8. DOI: 10.1111/j.1467-7652.2010.00521.x
Source: PubMed
15 Reads
  • Source
    • "Glycosylated CP of beet western yellows virus plays a role in the virus/aphis interaction and promotes the aphid transmission of the virus [46]. Reviewed earlier, plants offer an attractive alternative system for VLP vaccine production with cost-effective, scalable, versatile, appropriate glycosylation, efficient assembly of VLP, and safety from adventitious human pathogens [12]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Plants have been developed as an alternative system to mammalian cells for production of recombinant prophylactic or therapeutic proteins for human and animal use. Effective plant expression systems for recombinant proteins have been established with the optimal combination of gene expression regulatory elements and control of posttranslational processing of recombinant glycoproteins. In plant, virus-like particles (VLPs), viral "empty shells" which maintain the same structural characteristics of virions but are genome-free, are considered extremely promising as vaccine platforms and therapeutic delivery systems. Unlike microbial fermentation, plants are capable of carrying out N-glycosylation as a posttranslational modification of glycoproteins. Recent advances in the glycoengineering in plant allow human-like glycomodification and optimization of desired glycan structures for enhancing safety and functionality of recombinant pharmaceutical glycoproteins. In this review, the current plant-derived VLP approaches are focused, and N-glycosylation and its in planta modifications are discussed.
    BioMed Research International 05/2014; 2014(2):249519. DOI:10.1155/2014/249519 · 3.17 Impact Factor
  • Source
    • "Plant-based expression is a cost-effective and convenient system for antigens to be used in mucosal delivery. Plants represent relatively safe platforms to express vaccines since they are free of mammalian pathogens that affect other production systems such as transgenic animals and cell lines [29] [30]. Norwalk VLPs have been previously shown to accumulate in transgenic potato [18], tobacco [18] [21], and tomato [40] [47]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Narita 104 virus is a human pathogen belonging to the norovirus (family Caliciviridae) genogroup II. Noroviruses cause epidemic gastroenteritis worldwide. To explore the potential of developing a plant-based vaccine, a plant optimized gene encoding Narita 104 virus capsid protein (NaVCP) was expressed transiently in Nicotiana benthamiana using a tobacco mosaic virus expression system. NaVCP accumulated up to approximately 0.3 mg/g fresh weight of leaf at 4 days postinfection. Initiation of hypersensitive response-like symptoms followed by tissue necrosis necessitated a brief infection time and was a significant factor limiting expression. Transmission electron microscopy of plant-derived NaVCP confirmed the presence of fully assembled virus-like particles (VLPs). In this study, an optimized method to express and partially purify NaVCP is described. Further, partially purified NaVCP was used to immunize mice by intranasal delivery and generated significant mucosal and serum antibody responses. Thus, plant-derived Narita 104 VLPs have potential for use as a candidate subunit vaccine or as a component of a multivalent subunit vaccine, along with other genotype-specific plant-derived VLPs.
    BioMed Research International 05/2014; 2014:807539. DOI:10.1155/2014/807539 · 3.17 Impact Factor
  • Source
    • "Plants are promising biofactory systems for AMPs; they are economical to grow, easily scalable and generally regarded as safe because of the low risk of contamination with human and animal pathogens [10]. Plants have successfully been used for the production of different proteins for therapeutic and technological applications [11]. However, little attention has been paid to the use of plants as biofactories for AMPs. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Cecropin A is a natural antimicrobial peptide that exhibits rapid, potent and long-lasting lytic activity against a broad spectrum of pathogens, thus having great biotechnological potential. Here, we report a system for producing bioactive cecropin A in rice seeds. Transgenic rice plants expressing a codon-optimized synthetic cecropin A gene drived by an endosperm-specific promoter, either the glutelin B1 or glutelin B4 promoter, were generated. The signal peptide sequence from either the glutelin B1 or the glutelin B4 were N-terminally fused to the coding sequence of the cecropin A. We also studied whether the presence of the KDEL endoplasmic reticulum retention signal at the C-terminal has an effect on cecropin A subcellular localization and accumulation. The transgenic rice plants showed stable transgene integration and inheritance. We show that cecropin A accumulates in protein storage bodies in the rice endosperm, particularly in type II protein bodies, supporting that the glutelin N-terminal signal peptides play a crucial role in directing the cecropin A to this organelle, independently of being tagged with the KDEL endoplasmic reticulum retention signal. The production of cecropin A in transgenic rice seeds did not affect seed viability or seedling growth. Furthermore, transgenic cecropin A seeds exhibited resistance to infection by fungal and bacterial pathogens (Fusarium verticillioides and Dickeya dadantii, respectively) indicating that the in planta-produced cecropin A is biologically active. Rice seeds can sustain bioactive cecropin A production and accumulation in protein bodies. The system might benefit the production of this antimicrobial agent for subsequent applications in crop protection and food preservation.
    BMC Plant Biology 04/2014; 14(1):102. DOI:10.1186/1471-2229-14-102 · 3.81 Impact Factor
Show more