Article

Indirubin shows anti-angiogenic activity in an in vivo zebrafish model and an in vitro HUVEC model.

Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China.
Journal of ethnopharmacology (impact factor: 2.32). 09/2010; 131(2):242-7. DOI:10.1016/j.jep.2010.05.016 pp.242-7
Source: PubMed

ABSTRACT Indirubin is an active ingredient of the traditional Chinese medicine, Dang Gui Long Hui Wan, commonly used for the treatment of chronic myelocytic leukemia (CML) and other inflammatory conditions. These anti-leukemic and anti-inflammatory activities may be mediated by anti-angiogenic action. To investigate the anti-angiogenic activity of indirubin, we tested its inhibitory effect on blood vessel formation in zebrafish embryos and on endothelial cell proliferation in culture.
The anti-angiogenic activity of indirubin was tested using transgenic zebrafish embryos with fluorescent vasculature and human umbilical vein endothelial cells (HUVECs). Apoptosis was analyzed with a terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay.
Indirubin dose-dependently inhibited intersegmental vessel formation in zebrafish embryos. It also inhibited HUVEC proliferation by the induction of cellular apoptosis and cell-cycle arrest at the G0/G1 phase.
The anti-angiogenic activity of indirubin may partly contribute to its anti-leukemic and anti-psoriatic properties and may be valuable for the treatment of diseases with excessive angiogenesis. The zebrafish model of angiogenesis was further validated in this study.

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Keywords

active ingredient
 
anti-angiogenic action
 
anti-angiogenic activity
 
anti-psoriatic properties
 
Apoptosis
 
blood vessel formation
 
cellular apoptosis
 
chronic myelocytic leukemia
 
dUTP nick end labeling
 
endothelial cell proliferation
 
excessive angiogenesis
 
human umbilical vein endothelial cells
 
Indirubin dose-dependently inhibited intersegmental vessel formation
 
induction
 
inhibitory effect
 
terminal deoxynucleotidyl transferase
 
traditional Chinese medicine
 
transgenic zebrafish embryos
 
zebrafish embryos
 
zebrafish model