Elastase, α1-proteinase inhibitor, and interleukin-8 in pre-dialyzed and hemodialyzed patients with chronic kidney disease

3rd Department and Clinics of Pediatrics, Immunology and Rheumatology of Developmental Age, Medical University of Wroclaw, Wroclaw, Poland.
Pediatrics International (Impact Factor: 0.73). 10/2010; 52(5):735-43. DOI: 10.1111/j.1442-200X.2010.03162.x
Source: PubMed

ABSTRACT Neutrophil elastase in complex with α(1) -proteinase inhibitor (NE-α(1) PI) and interleukin (IL)-8 may serve as indicators of neutrophil activation and inflammatory stage. The aim of the study was to evaluate NE-α(1) PI, α(1)-PI, and IL-8 levels in the blood of patients with chronic kidney disease (CKD) undergoing hemodialysis (HD) or conservatively treated (CT). The influence of a single HD session on the investigated parameters was also assessed.
Blood samples were obtained from two groups of hemodialyzed patients (children/young adults [group HD1, n = 8] and adults [group HD2, n = 13]), as well as 13 CT patients and a group of healthy subjects. The proteins were measured using enzyme-linked immunosorbent assay or radial immunodiffusion.
There were no significant differences in NE-α(1) PI, α(1)-PI, and IL-8 concentrations between the HD1 and HD2 patients. The levels of NE-α(1) PI were considerably higher than normal in both groups of HD patients (before and after the HD session) and in the CT patients. Higher titers of NE-α(1) PI (P < 0.05) and α(1)-PI (P < 0.01) were obtained in the adults during the course of HD. Increased NE-α(1) PI was positively correlated with α(1)-PI. The serum concentration of IL-8 was significantly higher in the HD2 patients before and after dialysis than in the controls.
The data indicate that in CKD patients, neutrophils are highly activated both in the pre-dialyzed period and on regular HD. Contact with the dialysis membrane during HD causes a significant increase in blood NE-α(1) PI and α(1)-PI in adults, but not in children/young adults. NE-α(1) PI seems to be a much better indicator of an inflammatory state in CKD patients than free α(1)-PI or IL-8.

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Available from: Daria Augustyniak, Sep 27, 2015
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    • "Protease secretion from neutrophils has been linked to pathological processes of a variety of inflammatory diseases, such as different types of kidney disorders [15]. Enhanced activation of neutrophils with an associated protease release into the circulation has been noticed in patients with CRF [16]. Proteolytic cleavage and inactivation of the HGF protein leading to inhibited signaling through the HGF receptor, c-Met, has been found in patients with chronic non-healing wounds [17]. "
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