Anti-inflammatory effects of celecoxib in rat lungs with smoke-induced emphysema.
ABSTRACT Chronic airway inflammation is a characteristic feature of destructive cigarette smoking (CS)-induced lung disease, particularly in patients with emphysema. Celecoxib, a specific cyclooxygenase-2 (COX-2) inhibitor, is widely used to treat inflammation. However, the exact mechanisms underlying this drug's anti-inflammatory effects have not yet been determined in pulmonary emphysema. Here, we explore whether celecoxib attenuates CS-induced inflammation in rat lungs. Rats were exposed to smoke and received celecoxib via intragastric feeding daily for 20 wk. We found that celecoxib inhibited interalveolar wall distance and pulmonary inflammation in the lungs of CS-treated rats. Celecoxib inhibited serum NO production, iNOS, COX-2 expression, and PGE(2) production in CS-treated lung tissues. Our immunohistochemical data showed that CS-induced CD68 and COX-2 expression were inhibited by celecoxib. Furthermore, celecoxib attenuated the activation of phospho-IkappaBalpha and NF-kappaB in CS-treated rat lung. In addition, there was an inhibitory effect of celecoxib on the COX-2 expression and NF-kappaB activation in LPS-stimulated RAW 264.7 macrophages. Celecoxib also attenuated NF-kappaB activation in COX-2 siRNA-transfected RAW 264.7 macrophages. Thus, our findings suggest that the anti-inflammatory effects of celecoxib are mediated by its effects on NF-kappaB-regulated gene expression, which ultimately reduces the progression of CS-induced pulmonary emphysema.
Conference Proceeding: Applying a layered control architecture to a free-flying space camera[show abstract] [hide abstract]
ABSTRACT: Describes a prototype robot for space applications. The robot is the first generation of a free-flying robotic camera that will assist astronauts in constructing and maintaining the space station. The goal of the robot is to provide remote views to astronauts and ground controllers. The robot can autonomously move to locations on an air bearing table, can track targets and can plan paths. The robot contains a large collection of different technologies and this paper concentrates on a layered control architecture that integrates all robot functionsIntelligence and Systems, 1998. Proceedings., IEEE International Joint Symposia on; 06/1998