Article

A Microfluidic Platform for Characterization of Protein-Protein Interactions

M. Javanmard and F. Pease are with the Department of Electrical Engineering, Stanford University, Stanford, CA 94305 USA (
IEEE Sensors Journal (Impact Factor: 1.85). 08/2009; 9(8):883-891. DOI: 10.1109/JSEN.2009.2022558
Source: PubMed

ABSTRACT Traditionally, expensive and time consuming techniques such as mass spectrometry and Western Blotting have been used for characterization of protein-protein interactions. In this paper, we describe the design, fabrication, and testing of a rapid and inexpensive sensor, involving the use of microelectrodes in a microchannel, which can be used for real-time electrical detection of specific interactions between proteins. We have successfully demonstrated detection of target glycoprotein-glycoprotein interactions, antigen-antibody interactions, and glycoprotein-antigen interactions. We have also demonstrated the ability of this technique to distinguish between strong and weak interactions. Using this approach, it may be possible to multiplex an array of these sensors onto a chip and probe a complex mixture for various types of interactions involving protein molecules.

2 Followers
 · 
146 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this manuscript we describe an electronic label-free method for detection of target cells, which has potential applications ranging from pathogen detection for food safety all the way to detection of circulating tumor cells for cancer diagnosis. The nanoelectronic platform consists of a stack of electrodes separated by a 30nm thick insulating layer. Cells binding to the tip of the sensor result in a decrease in the impedance at the sensing tip due to an increase in the fringing capacitance between the electrodes. As a proof of concept we demonstrate the ability to detect Saccharomyces Cerevisae cells with high specificity using a sensor functionalized with Concanavalin A. Ultimately we envision using this sensor in conjunction with a technology for pre-concentration of target cells to develop a fully integrated micro total analysis system.
    Proceedings of SPIE - The International Society for Optical Engineering 02/2014; DOI:10.1117/12.2037966 · 0.20 Impact Factor
  • Sensors and Actuators B Chemical 03/2014; 193:918-924. DOI:10.1016/j.snb.2013.11.100 · 3.84 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Diagnosis of Phenylketonuria (PKU) in newborns is important because it can potentially help prevent mental retardation since it is treatable by dietary means. PKU results in phenylketonurics having phenylalanine levels as high as 2 mM whereas the normal upper limit in healthy newborns is 120 uM. To this end, we are developing a microfluidic platform integrated with a SERS substrate for detection of high levels of phenylalanine. We have successfully demonstrated SERS detection of phenylalanine using various SERS substrates fabricated using nanosphere lithography, which exhibit high levels of field enhancement. We show detection of SERS at clinically relevant levels.
    Proceedings of SPIE - The International Society for Optical Engineering 01/2014; DOI:10.1117/12.2040845 · 0.20 Impact Factor

Full-text (2 Sources)

Download
22 Downloads
Available from
Jun 29, 2014

Similar Publications