Prion Strain Mutation Determined by Prion Protein Conformational Compatibility and Primary Structure

Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky Medical Center, Lexington, KY 40536, USA.
Science (Impact Factor: 33.61). 05/2010; 328(5982):1154-8. DOI: 10.1126/science.1187107
Source: PubMed

ABSTRACT Prions are infectious proteins composed of the abnormal disease-causing isoform PrPSc, which induces conformational conversion of the host-encoded normal cellular prion protein PrPC to additional PrPSc. The mechanism underlying prion strain mutation in the absence of nucleic acids remains unresolved. Additionally, the frequency of strains causing chronic wasting disease (CWD), a burgeoning prion epidemic of cervids, is unknown. Using susceptible transgenic mice, we identified two prevalent CWD strains with divergent biological properties but composed of PrPSc with indistinguishable biochemical characteristics. Although CWD transmissions indicated stable, independent strain propagation by elk PrPC, strain coexistence in the brains of deer and transgenic mice demonstrated unstable strain propagation by deer PrPC. The primary structures of deer and elk prion proteins differ at residue 226, which, in concert with PrPSc conformational compatibility, determines prion strain mutation in these cervids.

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Available from: Candace K Mathiason, Sep 29, 2015
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    • "Supernatants were stored at −80 • C until tested for anti-PrP IgA. The CWD oral inoculum used consisted of brain homogenate from a CWD prion infected white-tailed deer, prepared as previously described, which resulted in 100% infection rates in previous trials and contained a mixture of strains CWD1 and CWD2 [24] [25]. "
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    ABSTRACT: Prion disease is a unique category of illness, affecting both animals and humans, in which the underlying pathogenesis is related to a conformational change of a normal, self-protein called PrP(C) (C for cellular) to a pathological and infectious conformer known as PrP(Sc) (Sc for scrapie). Bovine spongiform encephalopathy (BSE), a prion disease believed to have arisen from feeding cattle with prion contaminated meat and bone meal products, crossed the species barrier to infect humans. Chronic wasting disease (CWD) infects large numbers of deer and elk, with the potential to infect humans. Currently no prionosis has an effective treatment. Previously, we have demonstrated we could prevent transmission of prions in a proportion of susceptible mice with a mucosal vaccine. In the current study, white-tailed deer were orally inoculated with attenuated Salmonella expressing PrP, while control deer were orally inoculated with vehicle attenuated Salmonella. Once a mucosal response was established, the vaccinated animals were boosted orally and locally by application of polymerized recombinant PrP onto the tonsils and rectal mucosa. The vaccinated and control animals were then challenged orally with CWD-infected brain homogenate. Three years post CWD oral challenge all control deer developed clinical CWD (median survival 602 days), while among the vaccinated there was a significant prolongation of the incubation period (median survival 909 days; p=0.012 by Weibull regression analysis) and one deer has remained CWD free both clinically and by RAMALT and tonsil biopsies. This negative vaccinate has the highest titers of IgA in saliva and systemic IgG against PrP. Western blots showed that immunoglobulins from this vaccinate react to PrP(CWD). We document the first partially successful vaccination for a prion disease in a species naturally at risk. Copyright © 2014 Elsevier Ltd. All rights reserved.
    Vaccine 12/2014; 33(5). DOI:10.1016/j.vaccine.2014.11.035 · 3.62 Impact Factor
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    • "In many cases, different prion strains show differences in biochemical properties, such as protease resistance or denaturant sensitivity, which correlate with variation in pathology and the time course of disease [17,18,19,20,21,22]. However, in other cases, prion strains have been isolated that vary in pathology, yet remain biochemically indistinguishable, according to the levels of sensitivity available with current assays [23]. Moreover, while genetic polymorphisms in PrP bias the formation of particular conformations of PrPSc, a single primary sequence can propagate a multitude of distinct prion strains [14]. "
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    ABSTRACT: Prion strains (or variants) are structurally distinct amyloid conformations arising from a single polypeptide sequence. The existence of prion strains has been well documented in mammalian prion diseases. In many cases, prion strains manifest as variation in disease progression and pathology, and in some cases, these prion strains also show distinct biochemical properties. Yet, the underlying basis of prion propagation and the extent of conformational possibilities available to amyloidogenic proteins remain largely undefined. Prion proteins in yeast that are also capable of maintaining multiple self-propagating structures have provided much insight into prion biology. Here, we explore the vast structural diversity of the yeast prion [RNQ+] in Saccharomyces cerevisiae. We screened for the formation of [RNQ+] in vivo, allowing us to calculate the rate of spontaneous formation as ~2.96x10(-6), and successfully isolate several different [RNQ+] variants. Through a comprehensive set of biochemical and biological analyses, we show that these prion variants are indeed novel. No individual property or set of properties, including aggregate stability and size, was sufficient to explain the physical basis and range of prion variants and their resulting cellular phenotypes. Furthermore, all of the [RNQ+] variants that we isolated were able to facilitate the de novo formation of the yeast prion [PSI+], an epigenetic determinant of translation termination. This supports the hypothesis that [RNQ+] acts as a functional amyloid in regulating the formation of [PSI+] to produce phenotypic diversity within a yeast population and promote adaptation. Collectively, this work shows the broad spectrum of available amyloid conformations, and thereby expands the foundation for studying the complex factors that interact to regulate the propagation of distinct aggregate structures.
    PLoS ONE 10/2013; 8(10):e79582. DOI:10.1371/journal.pone.0079582 · 3.23 Impact Factor
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    • "Supernatants were stored at −80 • C until tested for anti-PrP IgA. The CWD oral inoculum used consisted of brain homogenate from a CWD prion infected white-tailed deer, prepared as previously described, which resulted in 100% infection rates in previous trials and contained a mixture of strains CWD1 and CWD2 [24] [25]. "
    Alzheimer's and Dementia 07/2012; 8(4):P403-P404. DOI:10.1016/j.jalz.2012.05.1109 · 12.41 Impact Factor
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