Article

Ethanolic cofermentation with glucose and xylose by the recombinant industrial strain Saccharomyces cerevisiae NAN-127 and the effect of furfural on xylitol production.

State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Bioresource technology (impact factor: 4.25). 09/2010; 101(18):7104-10. DOI:10.1016/j.biortech.2010.03.129 pp.7104-10
Source: PubMed

ABSTRACT Saccharomyces cerevisiae strain NAN-127 (2n, prototroph), which contains the xylose reductase-xylitol dehydrogenase (XR-XDH) xylose metabolic pathway was used for the cofermentation of glucose and xylose. Oxygen supply was the most important factor for xylose fermentation and pH 4.5 and a ventilation rate of 0.04 vvm were optimal. The xylose utilization ratio reached 0.655 at an initial xylose concentration of 50 gL(-1) and was 0.9 at an initial concentration of 20 gL(-1). Addition of furfural at late logarithmic phase as electron acceptor to a final concentration of 3.0 gL(-1) decreased the xylitol yield by 17% under micro-aeration conditions without inhibiting cell growth, but also without an increase in ethanol yield. The results are important to the application of strain NAN-127 in the lignocellulosic ethanol process.

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    Xiao-Jun Ji, He Huang, Zhi-Kui Nie, Liang Qu, Qing Xu, George T Tsao
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    Advances in biochemical engineering/biotechnology 01/2012; 128:199-224. · 1.64 Impact Factor
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Keywords

electron acceptor
 
ethanol yield
 
final concentration
 
glucose
 
initial concentration
 
initial xylose concentration
 
lignocellulosic ethanol process
 
logarithmic phase
 
micro-aeration conditions
 
Oxygen supply
 
Saccharomyces cerevisiae strain NAN-127
 
strain NAN-127
 
ventilation rate
 
XR-XDH
 
xylitol yield
 
xylose
 
xylose fermentation
 
xylose reductase-xylitol dehydrogenase
 
xylose utilization ratio
 

Xiaoran Zhang