Article

Unique multipotent cells in adult human mesenchymal cell populations

Department of Stem Cell Biology and Histology, Graduate School of Medicine, Tohoku University, Sendai 980-8575, Japan.
Proceedings of the National Academy of Sciences (Impact Factor: 9.81). 05/2010; 107(19):8639-43. DOI: 10.1073/pnas.0911647107
Source: PubMed

ABSTRACT We found adult human stem cells that can generate, from a single cell, cells with the characteristics of the three germ layers. The cells are stress-tolerant and can be isolated from cultured skin fibroblasts or bone marrow stromal cells, or directly from bone marrow aspirates. These cells can self-renew; form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency; and can differentiate into endodermal, ectodermal, and mesodermal cells both in vitro and in vivo. When transplanted into immunodeficient mice by local or i.v. injection, the cells integrated into damaged skin, muscle, or liver and differentiated into cytokeratin 14-, dystrophin-, or albumin-positive cells in the respective tissues. Furthermore, they can be efficiently isolated as SSEA-3(+) cells. Unlike authentic ES cells, their proliferation activity is not very high and they do not form teratomas in immunodeficient mouse testes. Thus, nontumorigenic stem cells with the ability to generate the multiple cell types of the three germ layers can be obtained through easily accessible adult human mesenchymal cells without introducing exogenous genes. These unique cells will be beneficial for cell-based therapy and biomedical research.

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Available from: Akira Niwa, Oct 09, 2014
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    • "However, the superiority of this method over the use of unfractionated cells in terms of the number and/or functionality of the generated IPCs was not provided. Recently, Kuroda and colleagues isolated what they defined as multilineage differentiating stress-enduring (Muse) cells cultured from skin fibroblasts or bone marrow stromal cells [40]. These cells were positive for both CD105, a mesenchymal cell marker, and SSEA-3, a human pluripotency marker. "
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    BioMed Research International 01/2015; 2015. DOI:10.1155/2015/575837 · 2.71 Impact Factor
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    • "BM-derived MSCs show poluripotency-differentiation properties (Kuroda et al., 2013). Interestingly, stress conditions could enrich the expression of SSEA-3 in cultured MSCs (Kuroda et al., 2010). Y. Kuroda et al. demonstrated that long-term trypsin incubation could increase the recovery of cell clusters containing pluripotency-associated markers and renamed these cells as " multi-lineage differentiating stress-enduring " (MUSE) cells. "
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