Article

Antimicrobial activity of chlorophyll-based solution on Candida albicans and Enterococcus faecalis

Revista Sul-brasiliera de Odontologia 01/2007;
Source: DOAJ

ABSTRACT Chlorophyll is a phytotherapic substance that presents curingproperties, however it is rarely used in Dentistry. Objective Objective Objective Objective Objective: to analyze the antimicrobial activity of a chlorophyll-based solution on isolates of Candida albicans and Enterococcus faecalis by agar dilution method.Material and Methods: oral isolates and Candida albicans referencestrain (ATCC 18804) and Enterococcus faecalis (ATCC 29212) wereincluded in the study. Chlorophyll extract was diluted in Sabourauddextrose or brain heart infusion (BHI) agar, according to the testingmicroorganism and poured in Petri plates, obtaining the finalconcentrations of 50%, 25%, 12.5%, 6%, 3% and 1.5%. C. albicansand E. faecalis strains were plated with the aid of a Steers’ inoculator.Plates were incubated at 37°C for 48 h for C. albicans and at 5% CO2 for E. faecalis. The experiments were performed in duplicate. Results:the results showed that the concentrations 50% and 25% chlorophyll extract were effective against C. albicans. E. faecalis grew in the presence of all the concentrations.Conclusion: chlorophyll-based solution presents effective antimicrobial activity of C. albicans but did not present any activity on E. faecalis.

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    ABSTRACT: To investigate the antibacterial activity of henna (Lawsonia inermis Linn) obtained from different regions of Oman against a wide array of micro-organisms. Fresh henna samples were obtained from different regions of Oman as leaves and seeds. 100 g fresh and dry leaves and 50 g of fresh and dry seeds were separately soaked in 500 mL of ethanol for three days, respectively, with frequent agitation. The mixture was filtered, and the crude extract was collected. The crude extract was then heated, at 48 °C in a water bath to evaporate its liquid content. The dry crude henna extract was then tested for its antibacterial activity using well-diffusion antibiotic susceptibility technique. Henna extracts were investigated for their antibacterial activity at different concentrations against a wide array of different micro-organisms including a laboratory standard bacterial strain of Pseudomonas aeruginosa (NCTC 10662) (P. aeruginosa) and eleven fresh clinical isolates of P. aeruginosa obtained from patients attending the Sultan Qaboos University Hospital (SQUH). 2-Hydroxy-p-Nathoqinone-Tech (2-HPNT, MW=174.16, C10H6O3) was included as control (at 50% concentration) along with the henna samples tested. Henna samples demonstrated antibacterial activity against all isolates but the highest susceptibility was against P. aeruginosa with henna samples obtained from Al-sharqyia region. Omani henna from Al-sharqyia region demonstrates high in vitro anti-P. aeruginosa activity compared with many henna samples from different regions of Oman.
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May 20, 2014