Antimicrobial activity of chlorophyll-based solution on Candida albicans and Enterococcus faecalis

Revista Sul-brasiliera de Odontologia 01/2007;
Source: DOAJ


Chlorophyll is a phytotherapic substance that presents curingproperties, however it is rarely used in Dentistry. Objective Objective Objective Objective Objective: to analyze the antimicrobial activity of a chlorophyll-based solution on isolates of Candida albicans and Enterococcus faecalis by agar dilution method.Material and Methods: oral isolates and Candida albicans referencestrain (ATCC 18804) and Enterococcus faecalis (ATCC 29212) wereincluded in the study. Chlorophyll extract was diluted in Sabourauddextrose or brain heart infusion (BHI) agar, according to the testingmicroorganism and poured in Petri plates, obtaining the finalconcentrations of 50%, 25%, 12.5%, 6%, 3% and 1.5%. C. albicansand E. faecalis strains were plated with the aid of a Steers’ inoculator.Plates were incubated at 37°C for 48 h for C. albicans and at 5% CO2 for E. faecalis. The experiments were performed in duplicate. Results:the results showed that the concentrations 50% and 25% chlorophyll extract were effective against C. albicans. E. faecalis grew in the presence of all the concentrations.Conclusion: chlorophyll-based solution presents effective antimicrobial activity of C. albicans but did not present any activity on E. faecalis.

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