Article

Protocol on induction of TMJ articular disc degeneration in rats by utilization of botulinum toxin.

Department of Anatomy, Cell Biology and Physiology and Biophysics, Institute of Biology, UNICAMP, Campinas, SP, Brazil. <>
Archives of oral biology (impact factor: 1.65). 07/2010; 55(7):530-4. DOI:10.1016/j.archoralbio.2010.03.018
Source: PubMed

ABSTRACT To develop a model of experimental degeneration in the articular disc of the TMJ of rats through the use of botulinum toxin that can be used in future studies of degenerative diseases on fibrocartilage.
Aiming at the above-mentioned objective, 12 Lewis male rats were used and divided into two groups: CG, control group and DG, group of animals to which the botulinum toxin was administered (6 units/kg). The morphological analysis was carried out utilizing histological cuts stained with hematoxyline-eosine, toluidine blue and Picrosirius; the biochemical analysis was made by SDS-polyacrylamide gel electrophoresis.
The DG showed peculiar characteristics regarding a degeneration joint disk, compatible with those described in literature as: reduction of cells number, general disorganization of cells direction and extracellular matrix, increase in glycosaminoglycans content and degradation of the tissue collagen.
Based on the morphological and biochemical results, it was concluded that the proposed degeneration model showed to be satisfactory for futures studies of injuries and fibrocartilage regeneration processes.

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Keywords

12 Lewis male rats
 
6 units/kg
 
biochemical results
 
degeneration joint disk
 
degenerative diseases
 
experimental degeneration
 
extracellular matrix
 
fibrocartilage regeneration processes
 
future studies
 
futures studies
 
general disorganization
 
glycosaminoglycans content
 
hematoxyline-eosine
 
peculiar characteristics
 
Picrosirius
 
proposed degeneration model
 
SDS-polyacrylamide gel electrophoresis
 
tissue collagen
 
TMJ
 
utilizing histological cuts stained