Rapid Influenza Antigen Test for Diagnosis of Pandemic (H1N1) 2009

California Department of Public Health, Richmond, California 94804, USA.
Emerging Infectious Diseases (Impact Factor: 6.75). 05/2010; 16(5):824-6. DOI: 10.3201/eid1605.091797
Source: PubMed

ABSTRACT The StudyThe California Department of Public Health (CDPH) supplied QuickVue Influenza test kits to clinicians participating in the Centers for Disease Control and Prevention (CDC) Sentinel Provider Influenza Surveillance Program. Sentinel providers performed the QuickVue Influenza test on a first respiratory specimen obtained from outpatients with influenza-like illness (fever >100°F and cough and/or sore throat) using the foam swab provided by QuickVue. Clinicians collected a second respiratory specimen using a sterile Dacron swab that was stored in viral transport media at 4°C for Performance of rapid antigen test compared with PCR in the diagnosis of pandemic (H1N1) 2009*Ct values were available for 389 specimens in which pandemic (H1N1) 2009 virus was detected by PCR; of these, the median influenza A PCR Ct value was 26 for 135 specimens with a negative rapid test result and 21 for 254 specimens with a positive rapid test result (pComparison of cycle threshold (Ct) values for pandemic (H1N1) 2009 real-time reverse transcription–PCR-positive specimens (n = 389) with negative (neg) and positive (pos) rapid antigen test (RT) results. Solid lines represent median value for ...Other smaller studies have found comparable sensitivities, but higher specificities, for rapid antigen tests for pandemic (H1N1) 2009. In a CDC study of 45 samples provided by state laboratories, the sensitivity of all rapid tests was 40%–69%, including 69% for QuickVue Influenza A+B (6). Others have found the QuickVue rapid tests to have sensitivities of 51%–63% and specificities of 99%–100% (7–9). During a large cluster of school outbreaks in New York, NY, USA, the sensitivity and specificity of the Binax NOW (Inverness Medical International, Bedford, UK) rapid test were 17.8% and 93.6%, respectively (10). As we found, positive rapid antigen test results in other studies also appear to correlate with higher concentrations of pandemic (H1N1) 2009 virus (6,11,12).

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