Reduced sympathetic neurite outgrowth on uterine tissue sections from rats treated with estrogen.
ABSTRACT In order to evaluate the contribution of substrate-bound factors to the extent and patterning of the sympathetic innervation of rat uterus following estrogen treatment, superior cervical ganglion explants from neonatal and adult ovariectomized rats were cultured on tissue sections of fresh frozen uterus from adult ovariectomized rats treated with estrogen or a vehicle. The main findings were: (1) neurite growth was greatly influenced by histological features of the underlying section; (2) on myometrial sections, neurites followed the orientation of the main axis of the longitudinally sectioned muscle cells; (3) neurites showed limited growth on transversally sectioned smooth muscle; (4) neuritic patterning was unaffected by a reduction in migrating ganglionic non-neuronal cells; (5) neurite outgrowth, but not non-neural cell migration, was markedly reduced on myometrial sections from rats treated with estrogen. These results suggest that adult myometrium continues to provide signals allowing the organotypic patterning and growth of sympathetic axons, that estrogen treatment modifies myometrial substrate properties so that it is less supportive for sympathetic neurite growth, and that adult sympathetic neurons retain their ability to recognize substrate-bound cues present in the myometrium. On endometrial sections, neurites formed radially symmetric halos, which were reduced in size on estrogen-treated endometrial substrates. Thus, changes in the neuritogenic capacity of the uterus underlie plasticity in uterine sympathetic nerves, and alterations in substrate-bound factors contribute to the diminished receptivity of the estrogenized uterus to its sympathetic innervation.
- SourceAvailable from: Bo Shen
Conference Paper: Meta-caching and meta-transcoding for server-side service proxy[Show abstract] [Hide abstract]
ABSTRACT: This paper proposes novel concepts of meta-caching and meta-transcoding which indicate that intermediate results (metadata) of service (e.g., video transcoding) sessions are cached and subsequent identical service requests are served through transcoding from the cached metadata. The proposed methods provide a foundation to achieving superior storage and computation resource balance at service proxies. It is primarily useful in reducing the computing load of service proxies at the server side. The paper further identifies the appropriate metadata and its characteristics for three types of video transcoding tasks. Simulations based on these characteristics validate that the proposed methods achieve superior computing load reduction on transcoding service proxies.Multimedia and Expo, 2003. ICME '03. Proceedings. 2003 International Conference on; 08/2003