Properties of WT and mutant hERG K+ channels expressed in neonatal mouse cardiomyocytes

Section of Cardiovascular Medicine, Departments of Medicine and Physiology, University of Wisconsin, Madison, Wisconsin, USA.
AJP Heart and Circulatory Physiology (Impact Factor: 3.84). 04/2010; 298(6):H1842-9. DOI: 10.1152/ajpheart.01236.2009
Source: PubMed


Mutations in human ether-a-go-go-related gene 1 (hERG) are linked to long QT syndrome type 2 (LQT2). hERG encodes the pore-forming alpha-subunits that coassemble to form rapidly activating delayed rectifier K(+) current in the heart. LQT2-linked missense mutations have been extensively studied in noncardiac heterologous expression systems, where biogenic (protein trafficking) and biophysical (gating and permeation) abnormalities have been postulated to underlie the loss-of-function phenotype associated with LQT2 channels. Little is known about the properties of LQT2-linked hERG channel proteins in native cardiomyocyte systems. In this study, we expressed wild-type (WT) hERG and three LQT2-linked mutations in neonatal mouse cardiomyocytes and studied their electrophysiological and biochemical properties. Compared with WT hERG channels, the LQT2 missense mutations G601S and N470D hERG exhibited altered protein trafficking and underwent pharmacological correction, and N470D hERG channels gated at more negative voltages. The DeltaY475 hERG deletion mutation trafficked similar to WT hERG channels, gated at more negative voltages, and had rapid deactivation kinetics, and these properties were confirmed in both neonatal mouse cardiomyocyte and human embryonic kidney (HEK)-293 cell expression systems. Differences between the cardiomyocytes and HEK-293 cell expression systems were that hERG current densities were reduced 10-fold and deactivation kinetics were accelerated 1.5- to 2-fold in neonatal mouse cardiomyocytes. An important finding of this work is that pharmacological correction of trafficking-deficient LQT2 mutations, as a potential innovative approach to therapy, is possible in native cardiac tissue.

Download full-text


Available from: Katherine Holzem, Jul 19, 2015
1 Follower
29 Reads
  • Source
    • "Since the rat neonatal cardiomyocytes may only transiently express the relevant channel, another approach is to use adenoviral or lentiviral vectors containing the cDNA of interest to infect the cells and allow adequate expression for study in a more native system. Comparing the behavior of wild type HERG and KCNQ1 channels with previously characterized deleterious mutants in rat neonatal myocytes has confirmed initial phenotypic characterization (Li et al., 2001; Lin et al., 2010). These groups found that the wild-type and mutant channel behaved generally the same as in cultured cells with some slight differences. "
    Cardiac Arrhythmias - New Considerations, 02/2012; , ISBN: 978-953-51-0126-0
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this communication, we present a universal read-out system, which can be used to decode polarimetric fiber-optic sensors based on highly birefringent fibers. All such sensors use the same sensing principle, relying upon the dependence of modal birefringence on different physical parameters. To register the measurand-induced phase changes between polarization modes, we use the coherence-addressing principle. This requires that the interrogated sensor be powered by a broadband source (superluminescent diode) and that the total optical path delay introduced by the sensor be balanced in the decoding interferometer. The system performance in decoding temperature, elongation and hydrostatic pressure sensor is demonstrated.
    Conference Record - IEEE Instrumentation and Measurement Technology Conference 01/2002; 2. DOI:10.1109/IMTC.2002.1007223
  • [Show abstract] [Hide abstract]
    ABSTRACT: As a major cardiac voltage-gated sodium channel isoform in the heart, the Nav1.5 channel is essential for cardiac action potential initiation and subsequent propagation throughout the heart. Mutations of Nav1.5 have been linked to a variety of cardiac diseases such as long QT syndrome (LQTs), Brugada syndrome, cardiac conduction defect, atrial fibrillation, and dilated cardiomyopathy. The mutagenesis approach and heterologous expression systems are most frequently used to study the function of this channel. This review focuses primarily on recent findings of Nav1.5 mutations associated with type 3 long QT syndrome (LQT3) in particular. Understanding the functional changes of the Nav1.5 mutation may offer critical insight into the mechanism of long QT3 syndrome. In addition, this review provides the updated information on the current progress of using various experimental model systems to study primarily the long QT3 syndrome.
    Pediatric Cardiology 03/2012; 33(6):943-9. DOI:10.1007/s00246-012-0303-y · 1.31 Impact Factor
Show more