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Phylogenetic analysis of enterohemorrhagic Escherichia coli O157, Germany, 1987-2008.

Christian Jenke, Dag Harmsen, Thomas Weniger, Jorg Rothganger, Eija Hyytia-Trees, Martina Bielaszewska, Helge Karch, Alexander Mellmann

Institute for Hygiene, Munster, Germany.

Journal Article: Emerging Infectious Diseases (impact factor: 6.79). 04/2010; 16(4):610-6. DOI: 10.3201/eid1604.091361

Abstract

Multilocus variable number tandem repeat analysis (MLVA) is a subtyping technique for characterizing human pathogenic bacteria such as enterohemorrhagic Escherichia coli (EHEC) O157. We determined the phylogeny of 202 epidemiologically unrelated EHEC O157:H7/H- clinical isolates through 8 MLVA loci obtained in Germany during 1987-2008. Biodiversity in the loci ranged from 0.66 to 0.90. Four of 8 loci showed null alleles and a frequency < or =44.1%. These loci were distributed among 48.5% of all strains. Overall, 141 MLVA profiles were identified. Phylogenetic analysis assigned 67.3% of the strains to 19 MLVA clusters. Specific MLVA profiles with an evolutionary persistence were identified, particularly within sorbitol-fermenting EHEC O157:H-.These pathogens belonged to the same MLVA cluster. Our findings indicate successful persistence of this clone.

Source: PubMed

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Keywords

19 MLVA clusters
 
Biodiversity
 
characterizing human pathogenic bacteria
 
EHEC
 
enterohemorrhagic Escherichia coli
 
evolutionary persistence
 
Multilocus variable number tandem repeat analysis
 
Phylogenetic analysis