Group B streptococci colonization in pregnant women: Risk factors and evaluation of the vaginal flora

Department of Pathology, Botucatu Medical School, São Paulo State University, UNESP, Distrito de Rubião Júnior, Botucatu, São Paulo 18618-970, Brazil.
Archives of Gynecology (Impact Factor: 1.36). 03/2010; 283(4):717-21. DOI: 10.1007/s00404-010-1439-8
Source: PubMed


To determine the prevalence of group B streptococci (GBS) in our population, and to assess the association between risk factors and vaginal flora with maternal rectovaginal colonization.
Samples were obtained from 405 patients between 35 and 37 weeks of gestation. Swabs from the vaginal and perianal regions were cultured in Todd Hewitt and subcultured in blood agar. Colonies suggestive of GBS were submitted to catalase and CAMP test. The vaginal flora was evaluated on Gram stain vaginal smears. Socio-demographic and obstetric data were obtained by designed form. Considering maternal GBS colonization as the response variable, a logistic regression model was fitted by the stepwise method with quantitative and qualitative explanatory variables.
The prevalence of GBS colonization was 25.4%. The most frequent vaginal flora abnormalities were cytolytic vaginosis (11.3%), followed by bacterial vaginosis (10.9%), candidosis (8.2%) and intermediate vaginal flora II (8.1%). Logistic regression analysis revealed that maternal age, number of sexual intercourse/week, occurrence of previous spontaneous abortion, presence of candidosis and cytolytic vaginosis were associated with streptococcal colonization.
The prevalence of GBS is high in pregnant women and is associated with sexual intercourse frequency, previous spontaneous abortion and the presence of candidosis or cytolytic vaginosis.

1 Follower
13 Reads
  • [Show abstract] [Hide abstract]
    ABSTRACT: Today, ultra-high throughput screening (uHTS) methods enable screening of 100,000 samples/day or more to meet the demands for speed, with assay volumes miniaturised to 10 μl or below to reduce reagent consumption. Various methods have been employed and are now implemented on a common instrumental platform (EVOscreen™ by Evotec Technologies, Hamburg), which allows efficient selection of the best read-out mode for particular assay types, or even multiplexing of methods. Fluorescence correlation spectroscopy (PCS) is sensitive to changes in diffusion as seen when a fluorescent ligand binds to a receptor protein, for example. Fluorescence intensity distribution analysis (FIDA) allows detection of changes in specific brightness which could be caused for example by quenching, fluorescence resonance energy transfer (FRET) or accumulation of fluorophores on particles with multiple binding sites. By extending this method to 2-channel detection (2D-FIDA) using a polarising beamsplitter, anisotropy changes can be monitored, which is often the method of choice for small-ligand binding assays. Finally, using pulsed laser sources and single-photon counting electronics, fluorescence lifetimes can be measured providing a robust read-out parameter in various assay formats. We will present examples of assays and screens based on these detection modes which include the most important target classes and cover a variety of assay types. Several examples for these assay types and their validation for uHTS will be discussed.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Our understanding of the bacterial species inhabiting the female genital tract has been limited primarily by our ability to detect them. Early investigations using microscopy and culture-based techniques identified lactobacilli as the predominant members of the vaginal microbiota and suggested that these organisms might serve a protective function at the mucosal surface. Improvements in cultivation techniques and the development of molecular-based detection strategies validated these early findings and enabled us to recognize that the microbiota of the female genital tract is much more complex than previously suspected. Disruption of the vaginal microbial community due to invasion of exogenous organisms or by overgrowth of one or more endogenous species has important health implications for both the mother and newborn.
    Seminars in Fetal and Neonatal Medicine 09/2011; 17(1):51-7. DOI:10.1016/j.siny.2011.08.006 · 3.03 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This study aimed to determine the frequency of Chlamydia trachomatis (CT) infection among high risk Brazilian women and evaluate its association with vaginal flora patterns. This was a cross-sectional study, performed in an outpatient clinic of Bauru State Hospital, São Paulo, Brazil. A total of 142 women were included from 2006 to 2008. Inclusion criteria was dyspareunia, pain during bimanual exam, presence of excessive cervical mucus, cervical ectopy or with three or more episodes of abnormal vaginal flora (AVF) in the previous year before enrollment. Endocervical CT testing was performed by PCR. Vaginal swabs were collected for microscopic assessment of the microbial flora pattern. Gram-stained smears were classified in normal, intermediate or bacterial vaginosis (BV), and recognition of Candida sp. morphotypes. Wet mount smears were used for detection of Trichomonas vaginalis and aerobic vaginitis (AV). Thirty-four of 142 women (23.9%) tested positive for CT. AVF was found in 50 (35.2%) cases. The most frequent type of AVF was BV (17.6%). CT was strongly associated with the presence of AV (n = 7, 4.9%, P = 0.018), but not BV (n = 25, 17.6%, P = 0.80) or intermediate flora (n = 18, 12.7%, P = 0.28). A high rate of chlamydial infection was found in this population. Chlamydia infection is associated with aerobic vaginitis.
    Archives of Gynecology 09/2011; 285(4):1013-8. DOI:10.1007/s00404-011-2085-5 · 1.36 Impact Factor
Show more

Similar Publications