Detection of Ralstonia Pickettii Bacteria in M9 Medium using Polymer Nanocomposite Sensors
ABSTRACT Detection of bacteria such as Ralstonia pickettii in high purity water (HPW) has gained increasing interest for researchers. Contamination problems in HPW in both pharmaceutical and semiconductor applications can lead to a reduction in performance and inefficiency in production chains leading to huge expense. Real time detection of these bacteria in HPW prior to use can allow corrective action to be taken sooner, by reallocating the water for repurification or utilization elsewhere thereby reducing industrial costs The aim of this study was to evaluate the responses of polymer nanocomposite sensors to increasing concentrations of Ralstonia pickettii bacteria. M9 minimal growth medium was used to grow the bacteria and three different concentrations were prepared by taking samples after 2 hours, 4 hours and 6 hours of growth. The sensors showed large sensitivity to each concentration of bacteria and produced a decrease in response over the growth cycle of the bacteria. These sensors demonstrated the ability to detect early stage actively growing cells in real time, which is of large benefit to HPW applications. This work has shown that these sensors can be used to detect both lag phase and late exponential and stationary phase growing cells in a minimal nutrient environment.
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ABSTRACT: The research and development of new electronic-nose applications in the biomedical field has accelerated at a phenomenal rate over the past 25 years. Many innovative e-nose technologies have provided solutions and applications to a wide variety of complex biomedical and healthcare problems. The purposes of this review are to present a comprehensive analysis of past and recent biomedical research findings and developments of electronic-nose sensor technologies, and to identify current and future potential e-nose applications that will continue to advance the effectiveness and efficiency of biomedical treatments and healthcare services for many years. An abundance of electronic-nose applications has been developed for a variety of healthcare sectors including diagnostics, immunology, pathology, patient recovery, pharmacology, physical therapy, physiology, preventative medicine, remote healthcare, and wound and graft healing. Specific biomedical e-nose applications range from uses in biochemical testing, blood-compatibility evaluations, disease diagnoses, and drug delivery to monitoring of metabolic levels, organ dysfunctions, and patient conditions through telemedicine. This paper summarizes the major electronic-nose technologies developed for healthcare and biomedical applications since the late 1980s when electronic aroma detection technologies were first recognized to be potentially useful in providing effective solutions to problems in the healthcare industry.Sensors 12/2011; 11(1):1105-76. DOI:10.3390/s110101105 · 2.25 Impact Factor