Impact of surfactants on pretreatment of corn stover. Bioresour Technol

Center for Environmental Research and Technology, Chemical and Environmental Engineering Department, Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507, USA.
Bioresource Technology (Impact Factor: 4.49). 03/2010; 101(15):5941-51. DOI: 10.1016/j.biortech.2010.03.003
Source: PubMed


Lignin in pretreated cellulosic biomass can non-productively adsorb cellulase, resulting in loss of a significant portion of this expensive protein. In addition, lignin interferes with the path for cellulase action, slowing down hydrolysis. Thus, the effectiveness of enzymatic hydrolysis of pretreated lignocellulosic biomass can be significantly enhanced if lignin is removed or effectively modified before adding enzymes. In this study, the enzymatic digestibilities of solids resulting from using the surfactants Tween-80, dodecylbenzene sulfonic acid, and polyethylene glycol 4000 during water-only or dilute acid pretreatment of corn stover at 140-220 degrees C were evaluated. All of these surfactants increased lignin removal during pretreatment and reduced non-productive binding of enzymes on the biomass surface, but Tween-80 increased enzymatic hydrolysis yields and enhanced total sugar recovery more than the other two. Surfactant pretreatment was found to improve lignin solubility, which could improve cellulose digestibility by reducing unproductive binding to enzyme, and also appeared to enhance performance by modifying the biomass surface.

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Available from: Charles E Wyman, Oct 03, 2015
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    • "Acid, alkaline, thermal, and ultra-sonication are the most familiar pretreatment methods for food waste [20] [21] [22] [23]. A promising approach for using surfactants, especially nonionic ones, to improve enzymatic hydrolysis of particulate organic matter is needed to achieve a high hydrogen production [24]. A surfactant has the ability to modify substrate structure and make it more accessible to enzymes [25]. "
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    ABSTRACT: The influence of surfactant addition on the hydrogen fermentative of the organic fraction of municipal solid waste was extensively investigated under thermophilic conditions (55 ± 2 °C) in batch cultures. The addition of Tween 80® (T80) and polyethylene glycol (PEG 6000®) substantially improved hydrogen yields (HYs) resulting in 109.9 ± 7.1 and 113.8 ± 7.7 mlH2/gCarb.initial at T80 and PEG 6000® concentrations not exceeding 2.8% and 16.7 g/L, respectively. A combination of 2.8% T80 with 1.7 g/L PEG 6000® achieved slightly higher HYs of 116.7 ± 5.2 mlH2/gCarb.initial. An artificial neural network model reliably represented the relationship between the surfactant concentration and hydrogen production with a correlation coefficient (R2) of 0.980. Microbial community analysis of the batches supplemented with 2.8% T80 and 1.7 g/L PEG 6000® showed the dominance of the hydrogen-producing bacteria Enterobacter, Escherichia, Buttiauxella, and Pantoea. The study confirms the potential of surfactant addition for H2 production from wastes containing organics in a particulate form.
    Applied Energy 07/2015; 149:272–282. DOI:10.1016/j.apenergy.2015.03.127 · 5.61 Impact Factor
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    • "The presence of surfactants during acid pretreatment enhanced the enzymatic hydrolysis of recovered solids (Qi et al. 2010; Qing et al. 2010). The addition of surfactants during pretreatment led to increased removal of lignin and made the biomass surface more hydrophilic. "
    Bioresources 11/2014; 9(1). DOI:10.15376/biores.9.1.1290-1302 · 1.43 Impact Factor
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    • "The surfactants increase positive interactions between the substrates and enzymes (Malmsten and VanAlstine, 1996; Kaar and Holtzapple, 1998; Eriksson et al., 2002). Surfactant addition enhances lignin removal because of hydrophobic interactions between lignin and surfactant (Qing et al., 2010). The mechanisms of surfactant on enzymatic hydrolysis improvement were thoroughly investigated by Eriksson et al. (2002). "
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    ABSTRACT: This study investigated a novel pretreatment method, as an essential step, for production of second generation bioethanol from sugarcane bagasse (SCB). Effect of tween 80 (TW) and polyethylene glycol 4000 (PEG) on SCB pretreatment was assessed using 1-butyl-3-methyl imidazolium chloride ([BMIM]Cl) as an ionic liquid (IL). Different concentrations of TW and PEG were used to determine the optimum concentration of surfactant for the highest percentage of cellulose conversion. TW and PEG increased lignin removal by 12.5% over the IL-only pretreated sample. The 3% (w/w) PEG showed a significant increase in enzymatic digestibility with an efficiency of 96.2% after 12h of hydrolysis; this was 23% higher than the efficiency of SCB pretreated with IL. The increase in digestibility of surfactant assisted IL pretreatment method can be attributed to the decrease in cellulose crystallinity, changes in the cellulose lattice, and delignification; which was confirmed by FT-IR, XRD and FE-SEM analysis.
    Bioresource Technology 06/2014; 169C:33-37. DOI:10.1016/j.biortech.2014.06.023 · 4.49 Impact Factor
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