Impact of surfactants on pretreatment of corn stover. Bioresour Technol

Center for Environmental Research and Technology, Chemical and Environmental Engineering Department, Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507, USA.
Bioresource Technology (Impact Factor: 4.49). 03/2010; 101(15):5941-51. DOI: 10.1016/j.biortech.2010.03.003
Source: PubMed


Lignin in pretreated cellulosic biomass can non-productively adsorb cellulase, resulting in loss of a significant portion of this expensive protein. In addition, lignin interferes with the path for cellulase action, slowing down hydrolysis. Thus, the effectiveness of enzymatic hydrolysis of pretreated lignocellulosic biomass can be significantly enhanced if lignin is removed or effectively modified before adding enzymes. In this study, the enzymatic digestibilities of solids resulting from using the surfactants Tween-80, dodecylbenzene sulfonic acid, and polyethylene glycol 4000 during water-only or dilute acid pretreatment of corn stover at 140-220 degrees C were evaluated. All of these surfactants increased lignin removal during pretreatment and reduced non-productive binding of enzymes on the biomass surface, but Tween-80 increased enzymatic hydrolysis yields and enhanced total sugar recovery more than the other two. Surfactant pretreatment was found to improve lignin solubility, which could improve cellulose digestibility by reducing unproductive binding to enzyme, and also appeared to enhance performance by modifying the biomass surface.

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    • "Acid, alkaline, thermal, and ultra-sonication are the most familiar pretreatment methods for food waste [20] [21] [22] [23]. A promising approach for using surfactants, especially nonionic ones, to improve enzymatic hydrolysis of particulate organic matter is needed to achieve a high hydrogen production [24]. A surfactant has the ability to modify substrate structure and make it more accessible to enzymes [25]. "
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    ABSTRACT: The influence of surfactant addition on the hydrogen fermentative of the organic fraction of municipal solid waste was extensively investigated under thermophilic conditions (55 ± 2 °C) in batch cultures. The addition of Tween 80® (T80) and polyethylene glycol (PEG 6000®) substantially improved hydrogen yields (HYs) resulting in 109.9 ± 7.1 and 113.8 ± 7.7 mlH2/gCarb.initial at T80 and PEG 6000® concentrations not exceeding 2.8% and 16.7 g/L, respectively. A combination of 2.8% T80 with 1.7 g/L PEG 6000® achieved slightly higher HYs of 116.7 ± 5.2 mlH2/gCarb.initial. An artificial neural network model reliably represented the relationship between the surfactant concentration and hydrogen production with a correlation coefficient (R2) of 0.980. Microbial community analysis of the batches supplemented with 2.8% T80 and 1.7 g/L PEG 6000® showed the dominance of the hydrogen-producing bacteria Enterobacter, Escherichia, Buttiauxella, and Pantoea. The study confirms the potential of surfactant addition for H2 production from wastes containing organics in a particulate form.
    Applied Energy 07/2015; 149:272–282. DOI:10.1016/j.apenergy.2015.03.127 · 5.61 Impact Factor
    • "Thus, the addition of β-glucosidase was required to completely hydrolyze cellulose into glucose (Ahamed and Vermette, 2008b). Several studies have reported that the addition of non-ionic surfactants could improve the enzymatic hydrolysis of pretreated biomass or reduce the amount of enzyme loading (Eriksson et al., 2002; Kristensen et al., 2007; Qing et al., 2010; Tu and Saddler, 2010). Since there are multiple factors affecting enzymatic hydrolysis, response surface methodology (RSM) was used to design the experiment and determine the optimal conditions for the desirable responses. "
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    ABSTRACT: Enzymatic hydrolysis is an essential step in the fermentative H2 production process of lignocellulosic biomass to convert cellulose and hemicellulose into fermentable sugars. The waste from disposable wooden chopsticks (DWC) was represented as wood waste and investigated in this study. In order to optimize the condition of the enzymatic hydrolysis of alkaline pretreated DWC, response surface methodology is an efficient experimental tool used to determine the optimal conditions of numerous variables. In the present study, cellulase dosage, β-glucosidase dosage, Tween 80, and hydrolysis time, were found to have a significant effect on enzymatic hydrolysis based on the Plackett-Burman design. These factors were subsequently investigated on the optimal levels by a central composite design, which was determined at 36 FPU/g pretreated DWC of cellulase, 53 CBU/g pretreated DWC of β-glucosidase, and 0.4 g/g pretreated DWC of Tween 80 for 105 h. Under optimal conditions, glucose and reducing sugar yields were 121.7 and 435.8 mg/g pretreated DWC, respectively. Furthermore, enzymatic hydrolysate was applied as a substrate for fermentative H2 production and obtained a yield of 27 mL H2/g pretreated DWC by an anaerobic mixed culture.
    Journal of Water and Environment Technology 01/2015; 13(2):153-166. DOI:10.2965/jwet.2015.153
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    • "The presence of surfactants during acid pretreatment enhanced the enzymatic hydrolysis of recovered solids (Qi et al. 2010; Qing et al. 2010). The addition of surfactants during pretreatment led to increased removal of lignin and made the biomass surface more hydrophilic. "
    Bioresources 11/2014; 9(1). DOI:10.15376/biores.9.1.1290-1302 · 1.43 Impact Factor
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