Article

[Dose effect of radiotherapy on drug resistance in esophageal cancer cell].

Thoracic Surgery Department, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 02/2010; 26(2):152-3,156.
Source: PubMed

ABSTRACT To investigate the dose effect of fractioned ionizing radiation on the expression of MDR1 in human esophageal cancer cells.
The mRNA and protein levels of MDR1 in esophageal cancer cell line EC9706 incubated in culture containing 150 micromol/L CoCl(2); pre- and post-irradiation were measured by quantitative reverse transcription-polymerase chain reaction (QRT-PCR) and Western blot analysis, respectively. The chemo-sensitivity was analyzed by MTT.
Comparing with the control group, hypoxia culture significantly induced MDR1 expression (P<0.05). After 20 fractions of irradiation, the MDR1 expression was significantly up-regulated in 2 Gy/d.f group (P<0.05), while obviously decreased in 1 Gy/d.f group (P<0.05). Consequently, the cell inhibitory rate was significantly reduced in 2 Gy/d.f group, but increased in 1 Gy/d.f group (P<0.05).
These results indicate that MDR1 is differentially modulated by different doses of fractionated radiation, which should be taken into account when combining radiotherapy chemotherapy for esophageal cancer patients.

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Keywords

20 fractions
 
cell inhibitory rate
 
different doses
 
dose effect
 
esophageal cancer cell line EC9706 incubated
 
esophageal cancer patients
 
fractioned ionizing radiation
 
human esophageal cancer cells
 
MDR1
 
MDR1 expression
 
pre-
 
protein levels
 
quantitative reverse transcription-polymerase chain reaction
 
radiotherapy chemotherapy
 
Western blot analysis
 

Guang-Jian Zhang