Occurrence of Tetracycline Resistance Genes in Aquaculture Facilities with Varying Use of Oxytetracycline

Department of Civil and Environmental Engineering, University of Wisconsin at Madison, 3204 Engineering Hall, 1415 Engineering Drive, Madison, WI 53706, USA.
Microbial Ecology (Impact Factor: 3.12). 03/2010; 59(4):799-807. DOI: 10.1007/s00248-009-9624-7
Source: PubMed


The contribution of human activities to environmental reservoirs of antibiotic resistance is poorly understood. The purpose of this study was to determine if oxytetracycline (OTC) use in aquaculture facilities increased the detection frequency (i.e., prevalence) of tetracycline resistance (tet(R)) genes relative to facilities with no recent OTC treatment. We used polymerase chain reaction to screen water and sediment from four noncommercial fish farms in northwestern Wisconsin for the presence of ten tet(R) determinants: tet(A), tet(B), tet(D), tet(E), tet(G), tet(M), tet(O), tet(Q), tet(S), and tet(W). Water from farms with recent OTC use had significantly higher tet(R) detection frequencies than did water from farms without recent OTC use, with prevalence in raceways and rearing ponds of farms with recent OTC use exceeding by more than twofold that of farms not using OTC. Effluent from all farms, regardless of treatment regime, had higher tet(R) detection frequencies than their corresponding influent for all genes, but the specific combinations of tet(R) genes detected in a sample were not different from their corresponding influent. Although OTC use was associated with the increased occurrence and diversity of tet(R) genes in water samples, it was not found to relate to tet(R) gene occurrence in sediment samples. Sediment samples from facilities with no recent OTC use had significantly higher frequencies of tet(R) gene detection than did samples from facilities with recent OTC use. All of the tet(R) genes were detected in both the medicated and nonmedicated feed samples analyzed in this study. These findings suggest that both OTC treatment in aquaculture facilities and the farms themselves may be sources of tet(R) gene introduction to the environment. To our knowledge, this is the first study to use genotypic and cultivation-independent methods to examine tet(R) gene occurrence associated with OTC use in aquaculture.

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    • "Health management in the aquaculture industry is a key factor for the successful production of aquatic animals and their products, which can be achieved by efficient disease management with the help of vaccines and effective formulation of extracts and emulsion preparations (Austin and Austin 1999; Thanigaivel et al. 2014; Thomas et al. 2014). Plant-based extracts are now being used to treat and control diseases in aquaculture instead of commercial antibiotics which cause resistance against the particular pathogenic bacteria (Mirand and Zemelman, 2002; Seyfried et al. 2010). Moreover, the regular consumption of antibiotic treated fishes by humans can lead to complications. "
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    ABSTRACT: Aquaculture health management demands a constant growth in the production by stabilizing economic loss and implementing various natural plant-based bio products for treating pathogenic bacteria. Pathogenic effects of the Citrobacter freundii isolated from fingerlings of Tilapia collected from a fish farm showed symptoms like tail necrosis, septicemia, hemorrhage, and reddening of the body. Antibacterial activity of C. freundii was tested against various medicinal plants. Of the three plants screened, Azadirachta indica (Neem) showed good antibacterial activity against C. freundii. The present study revealed that A. indica plant extract at a concentration of 150 mg/l in vivo can be used as an alternative to antibiotics for treating this bacterial infection. The use of medicinal plant extract-mediated treatment was found to be very effective in controlling this bacterial infection.
    Aquaculture 02/2015; 437. DOI:10.1016/j.aquaculture.2014.12.008 · 1.88 Impact Factor
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    • "Between 2006 and 2012, 7 notifications by the RASFF specifically reported the detection of antibiotics including β-lactams (penicillin-like) and chloramphenicol in milk and milk products [5]. Potential health risks due to the presence of antibiotic residues in food and the environment have been discussed for a long time [3] [6] [7] [8] [9] [10], frequently with a focus on the increasing occurrence of antibiotic resistant bacterial strains [10] [11] [12]. "

    Microbial pathogens and strategies for combating them: science, technology and education, Edited by A. Méndez-Vilas, 12/2013: chapter Detection of antibiotic residues in food – pitfalls and optimization of agar diffusion tests in comparison with commercial test kits.: pages 359-366.; Formatex, Spain.., ISBN: 978-84-939843-9-7
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    • "were found in tetracycline-resistant bacteria isolated from Chilean salmon farms (Miranda et al., 2003). Recently, Seyfried et al. (2010) "
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    Frontiers in Microbiology 08/2013; 4:233. DOI:10.3389/fmicb.2013.00233 · 3.99 Impact Factor
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