Fluorescence lifetime in cardiovascular diagnostics.

University of California, Davis, Department of Biomedical Engineering, Davis, California 95616, USA.
Journal of Biomedical Optics (Impact Factor: 2.75). 01/2010; 15(1):011106. DOI: 10.1117/1.3327279
Source: PubMed

ABSTRACT We review fluorescence lifetime techniques including time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) and fluorescence lifetime imaging microscopy (FLIM) instrumentation and associated methodologies that allow for characterization and diagnosis of atherosclerotic plaques. Emphasis is placed on the translational research potential of TR-LIFS and FLIM and on determining whether intrinsic fluorescence signals can be used to provide useful contrast for the diagnosis of high-risk atherosclerotic plaque. Our results demonstrate that these techniques allow for the discrimination of important biochemical features involved in atherosclerotic plaque instability and rupture and show their potential for future intravascular applications.

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Available from: Laura Marcu, Mar 24, 2014
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    • "In comparison, the lifetime values do not depend on the aforementioned distance, and thus can be used to detect changes in the composition of the plaque. This means that the three-dimensional reconstruction of the luminal surface shown in Fig. 8(a) for the first channel (390/40 nm) and Fig. 8(b) for the second channel (452/45 nm) can become a valuable asset towards the assessment of the composition of the plaque, as has been shown in previous studies of our group [6] [7], that might be difficult to achieve through intensity measurements. Fig. 7. "
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