Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean. BMC Plant Biol 10:41

United States Department of Agriculture-Agricultural Research Service, Plant Research Unit, St Paul, MN 55108, USA.
BMC Plant Biology (Impact Factor: 3.94). 03/2010; 10(1):41. DOI: 10.1186/1471-2229-10-41
Source: PubMed

ABSTRACT The nutritional and economic value of many crops is effectively a function of seed protein and oil content. Insight into the genetic and molecular control mechanisms involved in the deposition of these constituents in the developing seed is needed to guide crop improvement. A quantitative trait locus (QTL) on Linkage Group I (LG I) of soybean (Glycine max (L.) Merrill) has a striking effect on seed protein content.
A soybean near-isogenic line (NIL) pair contrasting in seed protein and differing in an introgressed genomic segment containing the LG I protein QTL was used as a resource to demarcate the QTL region and to study variation in transcript abundance in developing seed. The LG I QTL region was delineated to less than 8.4 Mbp of genomic sequence on chromosome 20. Using Affymetrix Soy GeneChip and high-throughput Illumina whole transcriptome sequencing platforms, 13 genes displaying significant seed transcript accumulation differences between NILs were identified that mapped to the 8.4 Mbp LG I protein QTL region.
This study identifies gene candidates at the LG I protein QTL for potential involvement in the regulation of protein content in the soybean seed. The results demonstrate the power of complementary approaches to characterize contrasting NILs and provide genome-wide transcriptome insight towards understanding seed biology and the soybean genome.

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Available from: James E Specht, Aug 16, 2015
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    • "doi:10.3835/plantgenome2015.04.0024 This genetic map interval has a corresponding physical distance of 8.4 Mb, from 24.54 Mb to 32.92 Mb on chromosome 20 (Bolon et al., 2010). Hwang et al. (2014) further narrowed down the candidate region to a 3 Mb region located between 27.6 Mb to 30.0 Mb on the same chromosome. "
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