Propionibacteria used as probiotics - A review

Dairy Science & Technology (Impact Factor: 1.6). 01/1995; DOI: 10.1051/lait:19954-534

ABSTRACT The investigation of probiotics has been very intensive during the last decades, con-centrating mainly on lactic acid and bifidobacteria. But there is also clear evidence that propionibacteria have probiotic effects. The probiotic influence is based on the production of propionic acid, bacteriocins, vitamin B12, better exploitation of fodder, growth stimulation of other beneficial bacteria and the ability to stay alive during gastric digestion. In Finland, large test series with piglets receiving Propionibacterium freudenreichii in their fodder have been performed. The growth promotant effect was significant and the fodder demand was clearly lower when compared with the control group. The bacterial concentration used was, on average, 2x109 cfu / g and the dose / animal 1-5 g / d. The mineral and trace element contents of a Propionibacterium freudenreichii-mass have also been studied. In other European countries, mixtures of propionibacteria and lactic acid / bifidobacteria have been used with positive results as probiotics for calves, Propionibacteria have also been investigated as human probiotics, especially in curing intestinal disorders of children and elderly people. The occurrence of lactic acid bacteria and propionibacteria in living food is very interesting as different kinds of this food type obviously act as probiotics. Thus, propionibacteria can be considered as potential probiotics requiring further research. Durant les dernières décennies, la recherche de probiotiques a été très intense, principalement sur les bactéries lactiques et les bifidobactéries. Mais il est également évident que les bactéries propioniques ont des effets probiotiques. Leur activité probiotique est basée sur la production d'acide propionique, de bactériocines, de vitamine B12, la meilleure utilisation des fourrages, la stimulation de la croissance des autres bactéries bénéfiques et la capacité à survivre au cours de la digestion gastrique. En Finlande, un test à grande échelle a été effectué sur des porcelets auxquels on incorporait Propionibacterium freudenreichii à la nourriture. L'effet sur la promotion de la croissance était significatif et la demande de fourrage était nettement plus basse comparée au groupe contrôle. La concentration bactérienne utilisée était en moyenne de 2 x 109 ufc / g et la dose par animal de 1-5 g / jour. Les teneurs en minéraux et oligoéléments d'une biomasse de Propionibacterium freudenreichii ont également été étudiées. Dans d'autres pays européens, des mélanges de bactéries propioniques et lactiques / bifidobactéries ont été utilisés comme probiotiques pour les veaux, avec des résultats positifs. Les bactéries propioniques ont aussi été utilisées et examinées comme probiotiques chez l'homme, spécialement pour soigner les désordres intestinaux des enfants et des personnes âgées. La présence de bactéries lactiques et de bactéries propioniques dans les aliments vivants est très intéressante puisque plusieurs sortes d'aliments de ce type agissent manifestement comme probiotiques. Les bactéries propioniques peuvent donc être considérées comme des probiotiques potentiels nécessitant de plus amples recherches.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Nowadays, there is an increasing concern for the public health about the consequences from the long and increased use of antibiotics in livestock production. The use of antibiotics in animal feed as growth promoters has been completely banned by the European Union since 2006, based on their possible negative effects on human and animal health. The removal of growth promoters has led to animal performance problems and a rise in the incidence of certain animal diseases. Thus, there is an urgent need to find alternatives to antibiotics, especially in EU. Due to the modern consumers’ concern about the potential development of antibiotic-resistant bacteria as well as at the same time the need to prevent economic losses of the farmers, alternatives to antibiotics has been developed to prevent the health problems and to improve the growth performance in farm animals. Owing to the full ban of antibiotic use in EU there is an urgent need to find alternatives to in-feed antibiotics. New strategies and commercial products must be developed to improve animal health and performance, based on their safety, efficacy and cost-effectiveness. The aim of this study is to summarize the beneficial effects of currently used alternatives to in-feed antibiotics, i.e. probiotics, prebiotics, organic acids, phytogenic compounds and zeolites on health and growth performance in farm animals (swine, poultry and ruminants).
    CAB Reviews Perspectives in Agriculture Veterinary Science Nutrition and Natural Resources 04/2013; 8(32). DOI:10.1079/PAVSNNR20138032
  • [Show abstract] [Hide abstract]
    ABSTRACT: Fermented milk products are part of the diet in many parts of the world and are consumed on a regular basis. Enhancing the nutritional and therapeutic properties of traditional fermented milks can improve the health and physiology of consumers. In the present study, dahi and yoghurt were prepared using buffalo milk. Bulk freeze-dried cultures of dahi and yoghurt, either with or without supplementation of Bifidobacterium bifidum I and Lactobacillus acidophilus R, were used to prepare these products. Probiotic properties such as antimicrobial activity in vitro, effect on body weight and serum cholesterol level in albino rats, and sensory properties of the products were evaluated. Among the products tested, probiotic yoghurt showed very strong inhibition (>16 mm diameter) against all the food-borne pathogens tested. Supplementation of probiotic cultures helped to improve the body weight of albino rats (Wister) after 30 days of feeding, with a weight gain of 123.33 and 129.33 g with probiotic yogurt and probiotic dahi, respectively. The serum cholesterol level of these rats decreased gradually, with a significant reduction of 2.63, 4.1 and 4.68 mg/100 ml at the end of 30 days in rats fed with yoghurt, probiotic dahi and probiotic yoghurt, respectively, indicating the hypocholesterolaemic effect of the probiotic cultures. No significant difference in sensory scores of dahi and yoghurt was observed with the supplementation of probiotic cultures. Thus, the present study indicated the probiotic potential of dahi and yoghurt prepared using selected probiotic strains.
    Annals of Microbiology 09/2011; 62(3). DOI:10.1007/s13213-011-0331-5 · 1.04 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Direct-fed microbials (DFM) are used to improve livestock health and performance. The effects of 2 DFM products, a blend of 3 Bacillus strains (DFMB) and a Propionibacteriumspp. (DFMP), on broiler performance, nutrient utilization, and immune responses were investigated. Day-old (n = 120) male broilers were divided into 24 groups of 5 birds and fed 3 wheat-based diets in mash form (8 groups per diet) from d 1 to 22. The control diet was fed without or with 7.5 × 10(4) cfu/g of either DFMB or DFMP. From d 19 to 21 fecal samples were collected for determination of total tract apparent retention (TTAR) of nutrients and AMEn. On d 21, feed intake and BW were determined. On d 22, 5 birds per treatment were killed by cervical dislocation to collect jejunal and ileal contents for determination of digesta viscosity and apparent ileal digestibility (AID) of nutrients, respectively, and ileum, cecal tonsil, and spleen tissues for Toll-like receptors (TLR) and cytokine expressions. Compared with the control, DFM did not affect BW gain and feed intake but DFMP reduced G:F (P < 0.01). Compared with the control (2,875 kcal/kg), birds fed on DFMB and DFMP had higher AMEn (2,979 and 2,916 kcal/kg, respectively; P < 0.05), whereas both DFM reduced the AID of DM (P < 0.001) and CP (P < 0.01). Furthermore, DFMP reduced TTAR of NDF (29.0 vs. 18.4%; P < 0.001), whereas both DFM increased TTAR of DM and fat (P < 0.001). Supplementing DFMP downregulated ileal expression of TLR-2b, IL-2, IL-4, IL-6, IL-10, and IL-13, whereas DFMB downregulated TLR-2b, IL-2, IL-4, and IL-6 in all 3 tissues, IL-10 in the spleen, and upregulated IL-13 in the spleen. In conclusion, the DFM did not improve performance but increased the AMEn of diet by possibly increasing DM and fat retention. Overall, both DFM showed an antiinflammatory effect in the ileum, but DFMB had more effects on local and systemic immunity than DFMP.
    Poultry Science 03/2014; 93(3):625-35. DOI:10.3382/ps.2013-03575 · 1.54 Impact Factor