Propionibacteria used as probiotics - A review

Dairy Science & Technology (Impact Factor: 1.6). 01/1995; 75(4-5). DOI: 10.1051/lait:19954-534

ABSTRACT The investigation of probiotics has been very intensive during the last decades, con-centrating mainly on lactic acid and bifidobacteria. But there is also clear evidence that propionibacteria have probiotic effects. The probiotic influence is based on the production of propionic acid, bacteriocins, vitamin B12, better exploitation of fodder, growth stimulation of other beneficial bacteria and the ability to stay alive during gastric digestion. In Finland, large test series with piglets receiving Propionibacterium freudenreichii in their fodder have been performed. The growth promotant effect was significant and the fodder demand was clearly lower when compared with the control group. The bacterial concentration used was, on average, 2x109 cfu / g and the dose / animal 1-5 g / d. The mineral and trace element contents of a Propionibacterium freudenreichii-mass have also been studied. In other European countries, mixtures of propionibacteria and lactic acid / bifidobacteria have been used with positive results as probiotics for calves, Propionibacteria have also been investigated as human probiotics, especially in curing intestinal disorders of children and elderly people. The occurrence of lactic acid bacteria and propionibacteria in living food is very interesting as different kinds of this food type obviously act as probiotics. Thus, propionibacteria can be considered as potential probiotics requiring further research. Durant les dernières décennies, la recherche de probiotiques a été très intense, principalement sur les bactéries lactiques et les bifidobactéries. Mais il est également évident que les bactéries propioniques ont des effets probiotiques. Leur activité probiotique est basée sur la production d'acide propionique, de bactériocines, de vitamine B12, la meilleure utilisation des fourrages, la stimulation de la croissance des autres bactéries bénéfiques et la capacité à survivre au cours de la digestion gastrique. En Finlande, un test à grande échelle a été effectué sur des porcelets auxquels on incorporait Propionibacterium freudenreichii à la nourriture. L'effet sur la promotion de la croissance était significatif et la demande de fourrage était nettement plus basse comparée au groupe contrôle. La concentration bactérienne utilisée était en moyenne de 2 x 109 ufc / g et la dose par animal de 1-5 g / jour. Les teneurs en minéraux et oligoéléments d'une biomasse de Propionibacterium freudenreichii ont également été étudiées. Dans d'autres pays européens, des mélanges de bactéries propioniques et lactiques / bifidobactéries ont été utilisés comme probiotiques pour les veaux, avec des résultats positifs. Les bactéries propioniques ont aussi été utilisées et examinées comme probiotiques chez l'homme, spécialement pour soigner les désordres intestinaux des enfants et des personnes âgées. La présence de bactéries lactiques et de bactéries propioniques dans les aliments vivants est très intéressante puisque plusieurs sortes d'aliments de ce type agissent manifestement comme probiotiques. Les bactéries propioniques peuvent donc être considérées comme des probiotiques potentiels nécessitant de plus amples recherches.

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    • "However, there is far less literature on the probiotic properties of propionibacteria than on the lactobacilli and bifidobacteria. Two book chapters (Jan et al. 2007; Ouwehand et al. 2004) and two earlier reviews (Mantere-Alhonen 1995; Vorobjeva et al. 1995) have reported on these potentialities. Recent evidence has been described in the literature but never reviewed. "
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    ABSTRACT: Probiotics have been the subject of intensive research, mainly focusing on bifidobacteria and lactic acid bacteria. However, there is evidence that dairy propionibacteria also display probiotic properties, which as yet have been underestimated. The aim of this paper is to review recent data which report probiotic characteristics of dairy propionibacteria and to distinctly organise them based on the experimental strategy employed: ranked from in vitro evidence to in vivo trials, which is a new approach. In addition to the selection criteria for probiotics in areas such as food safety, technological and digestive stress tolerance, many potential health benefits have been described which includemodulation of microbiota andmetabolic activity in the gut, modulation of intestinal motility and absorption, impact on intestinal inflammation, modulation of the immune system and potential modulation of risk factors for cancer development. The robust nature of dairy propionibacteria towards technological stresses should allow their future use in various fermented probiotic foods. Among the probiotic properties of dairy propionibacteria described in the literature, some of these properties are different from those reported for bifidobacteria and lactic acid bacteria. However, supplementation with dairy propionibacteria in randomised, placebo-controlled, double-blind human trials has mainly involved mixtures of propionibacteria with probiotic bacteria from other genera. Clinical studies involving the use of dairy propionibacteria alone are lacking. Such studies will allow the specifically observed health benefits to be attributed to dairy propionibacteria. This, in turn, will allow the investigation of the synergistic effects with other probiotic bacteria or beneficial food components.
    Dairy Science and Technology 02/2011; 91(1):1-26. DOI:10.1051/dst/2010032 · 1.09 Impact Factor
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    • "They are rod shaped, aerobic to facultative, gram (+), catalase (+), capsulated and have the ability to produce spores / endospores which other micro organisms are not able to do. Most bacilli are active and versatile producers of hydrolytic enzymes and consequently can utilize as food a wide variety of protein, carbohydrates, lipids, glycosides , alcohols and organic acids (Al honen, 1995). "
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    ABSTRACT: Water source is one of the gifts of nature. The main source of water is rivers, seas, lakes, ponds and also rainwater. Water contains many bacteria. Most of the bacteria are useful and some are harmful to living beings. Water is used for many life activities, irrigation and in industry. An alternative to risky and inconsistent wild fishing and ever raising demand for protein in the third-world countries, aquaculture developed as a source and the production/yield is increasing over the years. The main objective of this present investigations are to find safe and eco-friendly technique to medigate bacterial epidemic outbreak and make public water system useful for human and livestock consumption. To find different levels of bacterial counts in different water sources and suitable remedies with probiotics to reduce bacterial count to human and livestock required levels.
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    ABSTRACT: A bioassay-guided isolation method was developed with Aspergillus fumigatus and Rhodotorula mucilaginosa as target organisms. The bioassay was a dilution bioassay and was performed after each fractionation step. The isolation procedure was based on fractionation of cell-free supernatants using solid phase extraction (SPE) and reversed-phase high performance liquid chromatography (RP-HPLC). Cell-free supernatant was fractionated on a C18 SPE column, and the 95% aqueous acetonitrile fraction was further fractionated on a preparative HPLC C18 column. Fractions active in the bioassay were further separated using preparative HPLC with a C18 column or a porous graphitized carbon column. In the second HPLC step the conditions were optimised for each active fraction. The method was used for analysis of cell-free supernatants from strains of lactic acid bacteria (LAB), propionic acid bacteria (PAB), and uninoculated sodium lactate (SL) medium. The structures of the isolated antifungal metabolites were characterised using nuclear magnetic resonance (NMR), mass spectrometry (MS) and gas chromatography-mass spectrometry (GC-MS). The studies of LAB strains resulted in the isolation and identification of several antifungal diketopiperazines (DKPs); cyclo(L-Leu-cis-4-OH-D-Pro), cyclo(L-Leu-trans-4-OH-L-Pro), cyclo(L-Phe-cis-4-OH-D-Pro), cyclo(L-Phe-trans-4-OH-L-Pro) and cyclo(L-Phe-L-Pro), and 3-hydroxy fatty acids (3-OH-FAs); (R)-3-hydroxydecanoic acid, (R)-3-hydroxydodecanoic acid, (R)-3-hydroxytetradecanoic acid and 3-hydroxy-5-cis-dodecenoic acid, and D-3-phenyllactic acid (PLA) and L-3-PLA. The antifungal metabolites isolated or identified from dairy PAB were; cyclo(L-Phe-L-Pro) and cyclo(L-Ile-L-Pro) and D-3-PLA and L-3-PLA. From SL medium, used for cultivation of dairy PAB, seven antifungal peptides, His-Pro-Leu-Pro-Leu, Phe-Leu-Pro-Tyr-Pro, Gly-Pro-Phe-Pro-Ile, Gly-Pro-Phe-Pro-Leu, Gly-Pro-Phe-Pro-Leu-Val, Val-Tyr-Pro-Phe-Pro-Gly-Pro-Ile and Val-Ala-Pro-Phe-Gly-Val-Ala-Val-Phe-Gly were isolated and characterised by FAB-MS/MS. The minimal inhibitory concentration (MIC) against A. fumigatus was 20 mg/ml for cyclo(L-Phe-L-Pro) and cyclo(L-Ile-L-Pro), 25 µg/ml for 3-OH-dodecanoic acid, 100 µg/ml for 3-OH-decanoic acid and 7.5 mg/ml for PLA. A high performance liquid chromatography electrospray mass spectrometry (HPLC-ES-MS) method was developed for identification of DKPs in a complex cultivation medium. For determination of the absolute configuration of 3-OH-FAs a GC-MS method was developed. The method was based on the analysis of a 3-O-trimethylsilyl N-(S)-phenylethylamide derivatives of the isolated 3-OH-FAs.
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