Article

Protection against lethal Rift Valley fever virus (RVFV) infection in transgenic IFNAR(-/-) mice induced by different DNA vaccination regimens.

Centro de Investigación en Sanidad Animal (CISA-INIA), Ctra. Algete-El Casar, 28130 Valdeolmos, Madrid, Spain.
Vaccine (impact factor: 3.77). 02/2010; 28(17):2937-44. DOI:10.1016/j.vaccine.2010.02.018 pp.2937-44
Source: PubMed

ABSTRACT In this work, plasmid constructs encoding two different M segment ORFs, as well as the nucleoprotein N, have been used in different vaccination regimes to test protection against a RVFV-MP12 virus challenge in a transgenic mouse model with impaired interferon type I response (IFNAR(-/-)). We obtained dose dependent protection in animals immunized with a construct encoding both mature glycoproteins (pCMV-M4), whereas only partial protection in animals vaccinated with either N construct (pCMV-N) or a combination of both plasmids (pCMV-M4+pCMV-N). The protection elicited by the expression of the mature glycoproteins could be directly related to the induction of neutralizing antibodies against them. Interestingly, the combination of both vaccine constructs induced specific lymphoblast proliferation upon stimulation with a recombinant nucleoprotein.

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Keywords

construct encoding
 
different vaccination regimes
 
dose dependent protection
 
mature glycoproteins
 
neutralizing antibodies
 
partial protection
 
plasmids
 
protection elicited
 
RVFV-MP12 virus challenge
 
test protection
 
transgenic mouse model
 
vaccine constructs induced specific lymphoblast proliferation