Bioactive Steroidal Alkaloids from Sarcococca hookeriana

Institute of Forestry, Pokhara Campus, Tribhuvan University, Pokhara, Kaski, Nepal.
Planta Medica (Impact Factor: 2.15). 02/2010; 76(10):1022-5. DOI: 10.1055/s-0029-1240896
Source: PubMed


Four new 5 alpha-pregnane-type steroidal alkaloids, hookerianamides L(1), M(2), N(3), and O(4), and a known N-formylchonemorphine (5) have been isolated by acid-base extraction of the dichloromethane extract of Sarcococca hookeriana. The structures of all compounds were determined with spectroscopic techniques and by comparison with literature data. All compounds displayed antileishmanial and antibacterial properties. Compounds 1, 4, and 5 were found to be more potent than standard pentamidine (IC (50) = 9.59 microg/mL) with respect to leishmanicidal activity. The minimum inhibitory concentration of most of the compounds against Bacillus subtilis, Streptococcus minor, and Streptococcus ferus was lower than that of the standard ampicillin.

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    • "The dimethylamino group at C-3 was assigned an α-position based on the 13 C NMR chemical shift of C-3, and the chemical shift and coupling constant of H-3. The resonance of H-3 as a broad singlet indicated that it is equatorially oriented [7]; in the case of axial orientation it would appear as a multiplet with W 1/2 = >10, as reported in the literature [3]. These data were compared with those reported compound in which the stereochemistry was "
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    Natural product communications 09/2015; 10(9):1533. · 0.91 Impact Factor
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    • "The compounds responsible for their pharmacological effects are still unknown. Previous studies have shown that pregnane-type alkaloids are the major components of S. hookeriana and possess antibacterial [3], antileishmanial [3], antiplasmodial [4], and cholinesterase inhibitory activities [5]. Osteoarthritis is one of the main symptoms of rheumatism. "
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