Article

Sulfatides are associated with neointimal thickening after vascular injury

Department of Cardiovascular Medicine, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Tochigi, 321-0293 Japan.
Atherosclerosis (Impact Factor: 3.97). 02/2010; 211(1):291-6. DOI: 10.1016/j.atherosclerosis.2010.01.033
Source: PubMed

ABSTRACT Sulfatides are known to be a native ligand of P-selectin. Platelet-leukocyte interaction via the cross-talk between P-selectin and Mac-1 (CD11b/CD18) plays an important role in the mechanism of neointimal thickening after vascular injury such as that seen in post-stent restenosis. However, the roles of sulfatides on restenosis have not been elucidated.
Serum sulfatide levels, P-selectin expression on the surface of platelets, and activated Mac-1 on the surface of neutrophils were serially measured using both coronary sinus and peripheral blood samples in 21 patients who underwent coronary stent implantation.
The trans-cardiac gradient (coronary sinus minus peripheral blood) of the sulfatide levels significantly increased at 15 min (-1.47+/-2.87 to 0.59+/-1.44 nmol/ml, p<0.001), compared to baseline levels. The maximum response of the trans-cardiac gradient of P-selectin expression on the surface of platelets at 15 min after stent implantation (R=0.55, p<0.01), and that of activated Mac-1 on the surface of neutrophils at 48 h (R=0.59, p<0.01), were both positively correlated with that of serum sulfatide levels at 15 min. The angiographic late lumen loss was correlated with the trans-cardiac gradient of sulfatide levels at 15 min (R=0.48, p<0.05), platelet P-selectin expression at 15 min (R=0.42, p<0.05) and activated neutrophil Mac-1 expression at 48 h (R=0.46, p<0.05), but not with values at other sampling points.
Sulfatides may play a physiological role on inflammation in vascular injury and the development of neointimal thickening.

0 Followers
 · 
99 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: In this paper, we focus our study to follow systematically the influence of the magnetic field on the crystal form of calcium carbonate precipitated from low-concentration water solutions. By changing the strength of the field and the flow rate of the water through the system the calcite/aragonite/vaterite ratio varied. The crystal form and the particle size distribution of the precipitated calcium carbonate were determined by using X-ray analysis and HREM. The theoretical part of the work was to study the mechanism of the influence of magnetic field on the nucleation and further crystallisation of calcium carbonate. Starting from ab-initio calculations scaling can be explained easily on the basis of shape and energy position of the ground electronic states of calcite and aragonite.
  • [Show abstract] [Hide abstract]
    ABSTRACT: We investigated the changes in characteristics of neutrophil CD11b, monocyte CD11b, platelet CD62P, endothelin (ET), and neutrophil CD178 in patients with coronary heart disease (CHD) before and after primary coronary stenting. A total of 41 patients with CHD who underwent coronary stenting and 40 control subjects were enrolled in the study. In CHD patients, peripheral blood samples were taken 24 h before and 30 min, 24 h, and 72 h after successful coronary stenting. All markers were significantly elevated in patients with CHD compared with controls (P<0.05). Time-course studies revealed that the expressions of neutrophil CD11b, monocyte CD11b, platelet CD62P, and ET were lower at 30 min post-operation (PO) compared with that at 24 h before operation (BO) (P<0.05). All levels significantly increased from 30 min PO to 24 h PO (P<0.05) and decreased thereafter until 72 h PO (P>0.05). Time course changes in neutrophil CD11b levels after coronary stenting were significantly higher in patients with unstable angina pectoris than in patients with stable angina pectoris (P<0.05). CD11b levels were related to CD62P in patients with CHD (P<0.05). Neutrophil CD11b and monocyte CD11b levels were significantly increased in patients with CHD who underwent coronary stenting compared with controls (P<0.05). Results show that CD11b levels increased, meanwhile, the levels of CD62P and ET increased in CHD patients after coronary stenting. In addition, neutrophil CD178 levels of apoptosis factor in patients, which is important for regression of inflammation, remained high for a period of time after coronary stenting.
    International immunopharmacology 08/2011; 11(11):1850-4. DOI:10.1016/j.intimp.2011.07.015 · 2.71 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: This chapter contains sections titled: Introduction Lipids and Atherosclerotic Vascular Disease Diagnostics and Treatment Conclusions References
    Lipidomics, 10/2012: pages 251-267; , ISBN: 9783527330980
Show more