Resistance to antimicrobial agents of Campylobacter spp. strains isolated from animals in Poland.
ABSTRACT A total of 69 Campylobacter jejuni and 16 Campylobacter coli strains isolated from chicken, dog and pig stool samples were characterized based on their resistance to five antimicrobial agents and on plasmid pTet profiles. Antimicrobials used in this study were: amoxicillin/clavulanic acid, ciprofloxacin, erythromycin, tetracycline and trimethoprim/sulfamethoxazole. Among the isolates studied, 91.7% were resistant to one or more antimicrobial agent. The highest level of resistance for the whole test group was to trimethoprim/sulfamethoxazole (57.6%), followed by ciprofloxacin (44.2%) and tetracycline (20%). All isolates were susceptible to amoxicillin/clavulanic acid. Strains isolated from chickens were susceptible to erythromycin. Few erythromycin-resistant strains were isolated from dogs and pigs (5.8%). C. coli strains exhibited a higher antibiotic resistance than C. jejuni strains, excluding resistance to trimethoprim/sulfamethoxazole. The pTet plasmid harboring the tet(O) gene was detected in 14 Campylobacter spp. strains. Our studies demonstrate that the majority (71.4%) of tetracycline-resistant isolates carry a plasmid-borne tet(O) gene, particularly strains for which the minimum inhibitory concentration (MIC) are > or = 256 microg/ml. In conclusion, we have found high-level trimethoprim/sulfamethoxazole, ciprofloxacin and tetracycline resistance in Polish strains isolated from different sources. This study has demonstrated that resistance of Campylobacter species differs depending on both the bacterial species and animal origins. All strains that displayed resistance to four antimicrobial agents were isolated from pigs. Localization of the tet(O) gene on either plasmid or chromosome was not found to be correlated with tetracycline resistance.
- SourceAvailable from: Masood Ghane[show abstract] [hide abstract]
ABSTRACT: During the past decade Campylobacter has been shown to be responsible for enteritis in human and animal. The natural habitats of most Campylobacter species are the intestines of birds and other warm-blooded animals. These organisms may enter the environment, including drinking water, through the feces of animals, birds or infected humans. Fecal samples of Domestic Animals and Poultry were subjected to survey frequency of occurrence of pathogenic Campylobacter spp. in Tonekabon and Shiraz. Antimicrobial susceptibility of the isolates was assessed to evaluate the rate of antibiotic resistant campylobacter’s in both cities. The method for isolation of pathogenic Campylobacter spp. was Kapandis Baseri (prêt-KB) and for antimicrobial susceptibility of the isolates was disk diffusion and E-test. A total of 28 and 37 Campylobacter spp. were isolated in Tonekabon and Shiraz, respectively. All pathogenic Campylobacter spp. isolates were sensitive to Ciprofloxacin, however, varied responses to other antibiotics have been observed among the isolates. In addition, lowest MIC values were found for Ciprofloxacin and Gentamicin and highest MIC values were found for Erythromycin, Chloramphenicol, Gentamicin and Tetracycline. Overall, based on our observations, domestic animals and poultry should be considered as reservoirs of Campylobacter spp. in both cities. Although, frequency of existence of antibiotic resistance Campylobacter in Tonekabon was relatively high, Ciprofloxacin resistant Campylobacter were isolated neither from Tonekabon nor Shiraz. The Result obtained from data statistical analyses showed significant correlation (P<0.05) between the isolation rate of susceptible strains of Campylobacter to Cefalexin, Cefalotin and Ampicillin in Tonekabon and Shiraz.Journal of paramedical science. 01/2011; 2:21-26.