Hydroxyapatite nanorods/poly(vinyl pyrolidone) composite nanofibers, arrays and three-dimensional fabrics: electrospun preparation and transformation to hydroxyapatite nanostructures.

State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, People's Republic of China.
Acta biomaterialia (Impact Factor: 5.68). 02/2010; 6(8):3013-20. DOI: 10.1016/j.actbio.2010.02.015
Source: PubMed

ABSTRACT Electrospinning has been recognized as an efficient technique for fabricating polymer nanofibrous biomaterials. However, the study of electrospun inorganic biomaterials with well-designed three-dimensional (3-D) structures is still limited and little reported. In this study hydroxyapatite (HAp) nanorods with an average diameter of approximately 7 nm and length of approximately 27 nm were synthesized through a simple precipitation method and used for the fabrication of inorganic/organic [poly(vinyl pyrolidone) (PVP)] composite nanofibers by electrospinning in ethanol solution. 3-D fabrics and aligned nanofiber arrays of the HAp nanorods/PVP composite were obtained as precursors. Thereafter, 3-D single phase HAp fabrics, tubular structures and aligned nanofiber arrays were obtained after thermal treatment of the corresponding composite precursors. Cytotoxicity experiments indicated that the HAp fabric scaffold had good biocompatibility. In vitro experiments showed that mesenchymal stem cells could attach to the HAp fabric scaffold after culture for 24h.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Hydroxyapatite (HAP) nanostructures have wide potential applications in many fields such as drug delivery, tissue engineering, bone repair, gas sensing, catalysis and water treatment. Inspired with the fact that HAP has a high affinity with proteins, we have designed and developed a new synthetic strategy for three-dimensional (3-D) HAP nanosheet-assembled microspheres (HAP-NMSs) by employing hemoglobin as a soft template. The as-prepared products are characterized by X-ray powder diffraction (XRD) and field-emission scanning electron microscopy (SEM). The experimental results show that 3-D HAP microspheres are constructed by the self-assembly of HAP nanosheets as the building blocks. The influences of hemoglobin concentration, hydrothermal temperature and time on the morphology and crystal phase of the product are investigated. Based on the systematic investigation, a possible formation mechanism of HAP-NMSs is proposed. The as-prepared HAP-NMSs are explored for the potential application in water treatment. The experimental results indicate that the HAP-NMSs have a high adsorption capacity for heavy metal ions and selective adsorption activity for Pb2+ ions in acidic solution, thus are promising for the application in wastewater treatment.
    Journal of Colloid and Interface Science 01/2014; 416:11–18. · 3.55 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The present investigation reports the preparation of two types of 2D rod-like nano-hydroxyapatite (nHA) (unmodified and PPG wrapped) of varying high-aspect ratios, by modified co-precipitation methods, without any templates. These nHA were successfully introduced into novel synthesized Thermoplastic Polyurethane (TPU) matrices based on polycarbonate soft segments, by both in-situ and ex-situ techniques. Physico-mechanical properties of the in-situ prepared TPU/nHA nanocomposites were found to be superior compared to the ex-situ counterparts, and pristine nHA reinforced TPU. Improved biocompatibility of the prepared nanocomposites was confirmed by MTT assays using osteoblast-like MG63 cells. Cell proliferation was evident over an extended period. Osteoconductivity of the nanocomposites was observed by successful formation of an apatite layer on the surface of the samples, after immersion into simulated body fluid (SBF). Prothrombin time (PT) and activated partial thromboplastin time (APTT), as calculated from coagulation assays, displayed an increase in the clotting time, particularly for the PPG-wrapped nHA nanocomposites, prepared through the in-situ technique. Only 0.3% of hemolysis was observed for the in-situ prepared nanocomposites, which establishes the antithrombotic property of the material. The key parameters for enhancing the technical properties and biocompatibility of the nanocomposites are: the interfacial adhesion parameter (B�y ), the polymer-filler affinity, the aspect ratio of filler and non-covalent modifications, and the state of dispersion. Thus, the novel TPU/polymer wrapped nHA nanocomposites have great potential for biomedical applications, in particular for vascular prostheses, cardiovascular implants, scaffolds, and soft and hard tissues implants.
    Journal of Biomedical Nanotechnology 01/2014; · 7.58 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The multifunctional nanostructured materials hold the promise for the applications in future clinical treatment to enhance therapeutic efficacy. In the past several decades, various types of nanostructured materials with multifunctional properties have been developed and investigated for the biomedical applications. Although many achievements have been made, the design and preparation of novel multifunctional nanostructured biomaterials with well-defined structures, sizes and morphologies are still a long-term research direction. Calcium phosphate (CaP) nanostructured biomaterials with high biocompatibility are the ideal candidates for multifunctional nanosystems, and have been studied and applied in many biomedical fields such as bone repair/tissue engineering, drug/gene delivery. Compared with pure CaP materials, CaP based composite nanostructured materials, prepared by adding functional elements, molecules or other materials, have excellent properties and promising applications in hard tissue engineering, drug delivery, magnetic targeting and hyperthermia treatment for cancer, antibacterial, bioimaging, and so on. This review article is not intended to offer a comprehensive review on the research on CaP based multifunctional nanostructured materials and their applications in the biomedical fields, but shall present a brief summary of the recent progress in this exciting and rapidly evolving research field.
    Current Nanoscience 07/2014; 10(4). · 1.36 Impact Factor


Available from
Jun 3, 2014