Comparison of traditional phenotypic identification methods with partial 5' 16S rRNA gene sequencing for species-level identification of nonfermenting Gram-negative bacilli.

Joann L Cloud, Dag Harmsen, Peter C Iwen, James J Dunn, Gerri Hall, Paul Rocco Lasala, Karen Hoggan, Deborah Wilson, Gail L Woods, Alexander Mellmann

ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108, USA.

Journal Article: Journal of clinical microbiology (impact factor: 4.16). 02/2010; 48(4):1442-4. DOI: 10.1128/JCM.00169-10

Abstract

Correct identification of nonfermenting Gram-negative bacilli (NFB) is crucial for patient management. We compared phenotypic identifications of 96 clinical NFB isolates with identifications obtained by 5' 16S rRNA gene sequencing. Sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify NFB.

Source: PubMed

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Keywords

96 clinical NFB
 
assigned species
 
identifications
 
nonfermenting Gram-negative bacilli
 
patient management
 
phenotypic identifications
 
phenotypic results
 
sequencing results
 
usability