Gynostemma pentaphyllum protects mouse male germ cells against apoptosis caused by zearalenone via Bax and Bcl-2 regulation
College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, PR China. Toxicology mechanisms and methods
(Impact Factor: 1.52).
03/2010; 20(3):153-8. DOI: 10.3109/15376511003660185
The objective of this study was to explore the effects of Gynostemma pentaphyllum on Zearalenone-induced apoptosis in mouse male germ cells. Fifty Kunming male mice at 25-days-old were classified into five groups: group A was the control (10% ethanol, 0.5 ml/day); group B with 10 microg Zearalenone/day; group C with 10 microg Zearalenone and 50 mg/kg/day Gynostemma pentaphyllum; group D with 10 microg Zearalenone and 100 mg/kg/day Gynostemma pentaphyllum; and group E with 10 microg Zearalenone and 200 mg/kg/day Gynostemma pentaphyllum. It was found that Gynostemma pentaphyllum has a marked effect on protecting male germ cells against Zearalenone-induced apoptosis, as evidenced by a reduced apoptosis rate of male germ cells and Bax expression as well as an enhancement of Bcl-2 expression in Gynostemma pentaphyllum-treated groups compared to the control. In addition, Gynostemma pentaphyllum remarkably improved pathologic changes of testicular tissue, reduced the content of malondialdehyde (MDA), and increased the activity of superoxide dismutase (SOD) caused by Zearalenone. Taken together, these results suggest that Gynostemma pentaphyllum protects against toxicity caused by Zearalenone through anti-oxidation and anti-apoptosis via the regulation of Bax and Bcl-2 expression.
Available from: ajol.info
- "For hundreds of years, this herb has been commonly used in Chinese herbal medicines with the effects of clearing heat, detoxification, and as an anti-tussive and expectorant for relieving cough and chronic bronchitis (Zhang et al., 2005). A large number of pharmacological studies have demonstrated that GP possesses antimicrobial, anti-cancer, anti-aging, anti-fatigue, anti-ulcer, hypoglycemic, hypolipidemic and immunomodulatory qualities (Yeo et al., 2008; Yuan et al., 2010; Srichana et al., 2011; Im et al., 2012). GP has been shown to contain many biologically active phytochemicals like saponins, polysaccharides and flavonoids. "
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Gynostemma pentaphyllum (Thunb.) Makino has been reported to have a wide range of health benefits in Chinese herbal medicines. Polysaccharides from Gynostemma pentaphyllum (PGP), has been identified as one of the active ingredients responsible for its biological activities. Although many pharmacological activities of PGP have received a great deal of attention, there is limited evidence for the anti-fatigue effects of PGP. The purpose of this study was to investigate the effects of polysaccharides from PGP on physical fatigue.
Materials and method:
The rats were divided into four groups, with 10 animals per group: control (C), group, low-treated (LT), group, medium-treated (MT), group, and high-treated (HT), group. The C group received distilled water, while LT, MT and HT groups were given various doses of PGP (100, 200, 400 mg/kg· d). After 30 days, forced swimming test was carried out in an acrylic plastic pool, then the exhaustive swimming time of rats and some biochemical parameters related to fatigue were measured. The data obtained showed that PGP could extend the exhaustive swimming time of the rats, as well as decrease the blood lactic acid (BLA), and blood urea nitrogen (BUN), concentrations, and increase the hemoglobin, liver glycogen and muscle glycogen concentrations.
The data obtained showed that different doses of PGP could extend the exhaustive swimming time of the rats, as well as decrease the BLA and BUN concentrations, and increase the hemoglobin, liver glycogen and muscle glycogen concentrations, which suggests that PGP had significant anti-fatigue effects on rats.
PGP may be of use as a potential anti-fatigue agent, but there is a need for further research on long-term use in order to show its positive effects on physical fatigue.
African Journal of Traditional, Complementary and Alternative Medicines 07/2014; 11(3):112-7. DOI:10.4314/ajtcam.v11i3.17 · 0.56 Impact Factor
Available from: Fatima Elzeinova
- "These results show that a single dose of ZEA induces testicular germ cell apoptosis in a time-dependent and stage-specific manner in the rat testis in vivo. The induction of apoptosis in testicular tissue after ZEA treatment was also shown by Yuan et al. ; these authors have additionally shown that traditional medicinal plant Gynostemma pentaphyllum protects against toxicity caused by ZEA through anti-oxidation and anti-apoptosis effects mediated by the regulation of Bax and Bcl-2 expression. Filipiak et al.  performed an in vivo study of pubertal rats in which they investigated the effect of two xenoestrogens, diethylstilbestrol (DES) and ZEA, with comparison of their effect with natural oestrogen 17␤-estradiol (E2). "
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ABSTRACT: We tested the effect of two different concentrations (150μg/l and 0.15μg/l) of mycotoxin zearalenone (ZEA) on the reproductive parameters and expression of testicular genes in male mice. In adult males, no reduction of body or reproductive organ weight was observed, and the seminiferous tubules were morphologically normal with ongoing spermatogenesis. However, we found decreased sperm concentration, increase of morphologically abnormal spermatozoa and increased binding of apoptotic marker annexin V. This study was also focused on the evaluation of gene expression profiles of 28 genes playing important roles during the processes occurring in the testicular tissue. We detected changes in the expression of genes important for proper spermatogenesis. Surprisingly, we observed a stronger effect after exposure to the lower dose of ZEA.
Reproductive Toxicology 06/2014; 45:20-30. DOI:10.1016/j.reprotox.2014.01.003 · 3.23 Impact Factor
Available from: Yan-Jun Hou
- "SOD, an important intracellular antioxidant enzyme detoxifying superoxide anion, provides a significant protection against the oxidative effect of mycotoxin contaminated maize in comparison with the GPx and CAT. The SOD activity reduced in the testicular tissue of the ZEN-treated male mice , . While CAT has two enzymatic functions, not only catalyzing the breakdown of H2O2 into O2 and H2O, but also catalyzing the oxidation of electron donors such as ethanol or phenols in the presence of low concentrations of H2O2.In our present study, SOD activities in the liver and kidney were reduced, accompanied by the decreased CAT activity in the serum and kidney. "
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ABSTRACT: Mycotoxins which mainly consist of Aflatoxin (AF), Zearalenone (ZEN) and Deoxynivalenol (DON) are commonly found in many food commodities. Although each component has been shown to cause liver toxicity and oxidative stress in several species, there is no evidence regarding the effect of naturally contained multiple mycotoxins on tissue toxicity and oxidative stress in vivo. In the present study, mycotoxins-contaminated maize (AF 597 µg/kg, ZEN 729 µg/kg, DON 3.1 mg/kg maize) was incorporated into the diet at three different doses (0, 5 and 20%) to feed the mice, and blood and tissue samples were collected to examine the oxidative stress related indexes. The results showed that the indexes of liver, kidney and spleen were all increased and the liver and kidney morphologies changed in the mycotoxin-treated mice. Also, the treatment resulted in the elevated glutathione peroxidase (GPx) activity and malondialdehyde (MDA) level in the serum and liver, indicating the presence of the oxidative stress. Moreover, the decrease of catalase (CAT) activity in the serum, liver and kidney as well as superoxide dismutase (SOD) activity in the liver and kidney tissue further confirmed the occurrence of oxidative stress. In conclusion, our data indicate that the naturally contained mycotoxins are toxic in vivo and able to induce the oxidant stress in the mouse.
PLoS ONE 03/2013; 8(3):e60374. DOI:10.1371/journal.pone.0060374 · 3.23 Impact Factor
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