Glycoprofiling of the Human Salivary Proteome

Department of Chemistry and Biochemistry, University of California, Los Angeles, CA.
Clinical Proteomics 03/2009; 5(1):52-68. DOI: 10.1007/s12014-008-9021-0
Source: PubMed


Glycosylation is important for a number of biological processes and is perhaps the most abundant and complicated of the known post-translational modifications found on proteins. This work combines two-dimensional polyacrylamide gel electrophoresis (2-DE) and lectin blotting to map the salivary glycome, and mass spectrometry to identity the proteins that are associated with the glycome map. A panel of 15 lectins that recognize six sugar-specific categories was used to visualize the type and extent of glycosylation in saliva from two healthy male individuals. Lectin blots were compared to 2-D gels stained either with Sypro Ruby (protein stain) or Pro-Q Emerald 488 (glycoprotein stain). Each lectin shows a distinct pattern, even those belonging to the same sugar-specific category. In addition, the glycosylation profiles generated from the lectin blots show that most of the salivary proteins are glycosylated and that the pattern is more widespread than is demonstrated by the glycoprotein stained gel. Finally, the co-reactivity between two lectins was measured to determine the glycan structures that are most and least often associated with one another along with the population variation of the lectin reactivity for 66 individuals.

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Available from: Melissa Sondej, Jun 04, 2014
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    • "In order to identify potential biomarkers of health and disease in saliva, a complete profile of the proteins detectable, such as that provided by proteomic methods, is required (Hu et al., 2005; Guo et al., 2006; Sondej et al., 2009). In this study, we assessed the potential of 1-and 2-DE to separate porcine salivary proteins. "
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    ABSTRACT: Saliva contains a number of proteins that may be useful as biomarkers of health and disease and can be easily obtained from large numbers of animals in a non-invasive, stress-free way. The objective of this study was to explore the protein composition of porcine saliva from 10 specific pathogen free pigs using first one-dimensional SDS-PAGE and then two-dimensional electrophoresis and mass spectrometry. A reference proteome pattern for porcine saliva was established with the identification of 13 different, mainly saliva-specific, proteins. These reference data will facilitate the investigation of salivary proteins potentially altered in disease and could serve as novel diagnostic biomarkers.
    The Veterinary Journal 03/2011; 187(3):356-62. DOI:10.1016/j.tvjl.2009.12.020 · 1.76 Impact Factor
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    • "Indeed, the glycosylation profiles generated from lectin blots indicated that the majority of salivary proteins are potentially glycosylated (Sondej et al., 2009). Previous estimates established that immunoglobulins contribute from 5 to 15% of the total number of salivary proteins. "
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    ABSTRACT: The protein compositions, or the proteomes, found in human salivary and plasma fluids are compared. From recent experimental work by many laboratories, a catalogue of 2290 proteins found in whole saliva has been compiled. This list of salivary proteins is compared with the 2698 proteins found in plasma. Approximately 27% of the whole-saliva proteins are found in plasma. However, despite this apparent low degree of overlap, the distribution found across Gene Ontological categories, such as molecular function, biological processes, and cellular components, shows significant similarities. Moreover, nearly 40% of the proteins that have been suggested to be candidate markers for diseases such as cancer, cardiovascular disease, and stroke can be found in whole saliva. These comparisons and correlations should encourage researchers to consider the use of saliva to discover new protein markers of disease and as a diagnostic non-proximal fluid to detect early signs of disease throughout the body.
    Journal of dental research 10/2010; 89(10):1016-23. DOI:10.1177/0022034510380414 · 4.14 Impact Factor
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    ABSTRACT: Salivary diagnostics is a dynamic and emerging field utilizing nanotechnology and molecular diagnostics to aid in the diagnosis of oral and systemic diseases. In this article the author critically reviews the latest advances using oral biomarkers for disease detection. The use of oral fluids is broadening perspectives in clinical diagnosis, disease monitoring, and decision making for patient care. Important elements determining the future possibilities and challenges in this field are also discussed.
    Dental clinics of North America 01/2011; 55(1):159-78. DOI:10.1016/j.cden.2010.08.004
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