Article

Long-term frozen storage of choanoflagellate cultures.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA.
Cold Spring Harbor Protocols (impact factor: 4.63). 02/2009; 2009(2):pdb.prot5149. DOI:10.1101/pdb.prot5149 pp.pdb.prot5149
Source: PubMed

ABSTRACT INTRODUCTION Choanoflagellates are heterotrophic nanoflagellates: small, colorless protozoa that are present in marine and freshwater environments as well as in hydrated soils. Because they are the closest living relatives of the metazoa, the study of their cell biology and genomes promises to provide new insights into metazoan ancestry and origins. Choanoflagellates can be stored under stable conditions by freezing them with liquid nitrogen, as described here. To prevent cross-contamination among vials stored in the liquid phase of a liquid nitrogen freezer, the use of CryoFlex tubing to fully seal the vials is encouraged.

0 0
 · 
0 Bookmarks
 · 
42 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

cell biology
 
Choanoflagellates
 
closest
 
cross-contamination
 
freezing
 
freshwater environments
 
INTRODUCTION Choanoflagellates
 
liquid nitrogen freezer
 
liquid phase
 
metazoan ancestry
 
new insights
 
origins
 
small
 
stable conditions
 

Nicole King