Biodegradation of the major color containing compounds in distillery wastewater by an aerobic bacterial culture and characterization of their metabolites

Environmental Microbiology Section, Indian Institute of Toxicology Research , Lucknow, Uttar Pradesh, India.
Biodegradation (Impact Factor: 2.34). 02/2010; 21(5):703-11. DOI: 10.1007/s10532-010-9336-1
Source: PubMed

ABSTRACT This study deals the biodegradation of the major color containing compounds extracted from distillery wastewater (DWW) by an aerobic bacterial consortium comprising Bacillus licheniformis (DQ79010), Bacillus sp. (DQ779011) and Alcaligenes sp. (DQ779012) and characterization of metabolic products. The degradation of color containing compounds by bacteria was studied by using the different carbon and nitrogen sources at different environmental conditions. Results revealed that the bacterial consortium was efficient for 70% color removal in presence of glucose (1.0%) and peptone (0.1%) at pH 7.0 and temperature 37 degrees C. The HPLC analysis of control and bacterial degraded samples has shown the reduction in peak area as well as shifting of peaks compared to control indicating the bacterial degradation as well as transformation of color containing compounds from DWW. The comparative LC-MS-MS and other spectrophotometric analysis has shown the presence of dihydroxyconiferyl alcohol, 2, 2'-bifuran-5-carboxylic acid, 2-nitroacetophenone, p-chloroanisol, 2, 3-dimethyl-pyrazine, 2-methylhexane, methylbenzene, 2, 3-dihydro-5-methylfuran, 3-pyrroline, and acetic acid in control samples that were biodegraded and biotransformed into 2-nitroacetophenone, p-chloroanisol, 2, 2'-bifuran, indole, 2-methylhexane, and 2, 3-dihydro-5-methylfuran by bacterial consortium. In this study, it was observed that most of the compounds detected in control samples were diminished from the bacterial degraded samples and compounds 2, 2'-bifuran and indole with molecular weight 134 and 117 were produced as new metabolites during the bacterial degradation of color containing compounds from DWW.

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Available from: Ram Naresh Bharagava, Dec 17, 2013
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    • "Various methods have been tried to remove melanoidin color and recalcitrant COD. Biological processes employing bacteria [9], fungi [10], and enzyme [11] have been tried before to treat melanoidin-rich wastewater. Though biological processes are generally less costly than chemical or physical treatment methods, none of the reported methods can effectively remove the contaminants in melanoidin-rich wastewaters. "
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    ABSTRACT: Melanoidin-rich industrial effluents, e.g. from coffee extraction plants and molasses distilleries, can cause potential environmental problems due to the high content of remnant dissolved organic carbon and dark color. It mainly consists of melanoidins and other organic colorants, which are recalcitrant to biological treatment. The current study was aimed to develop a polishing step after anaerobic digestion for the colorant elimination from melanoidin-rich wastewater (molasses distillery wastewater, MDW) using natural manganese oxides. Anaerobically digested MDW was used to test the removal of organic contents and color at different pH values. It was observed that the kinetics of colorant elimination was best described by the second order equation, with a significant dependence on pH. Furthermore, the liquid chromatography with organic carbon detection was applied to analyze the changes in molecular composition during the reaction. There was a preferential removal of low weight melanoidin molecules over higher weight molecules.
    Separation and Purification Technology 08/2015; 150:286-291. DOI:10.1016/j.seppur.2015.07.013 · 3.09 Impact Factor
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    • "). Most of the metabolic products have been also reported from distillery effluent by fungal treatment (Gonzalez et al., 2000) and also as degradation products of synthetic and natural melanoidin after bacterial degradation as published in earlier studies (Bharagava and Chandra, 2010; Chandra et al., 2009). "
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    ABSTRACT: This study deals with the characterization of rhizosphere bacterial communities and metabolic products produced during the two stage sequential treatment of post methanated distillery effluent by bacteria and constructed wetland plants. Results showed that bacterial treatment followed by wetland plants (Phragmites cummunis) resulted 94.5% and 96.0% reduction in BOD and COD values, respectively. The PCR-RFLP analysis showed the presence of Stenotrophomonas, Enterobacter, Pantoea, Acinetobacter and Klebsiella sp., as dominant rhizosphere bacterial communities which play an important role in degradation and decolorization of PMDE in wetland treatment system. Further, the LC-MS-MS and other spectrophotometric analysis have shown that most of the pollutants detected in untreated PMDE were diminished from bacteria and wetland plant treated PMDE indicating that bacteria and wetland plant rhizosphere microbes utilized them as carbon, nitrogen and energy source. While, methylbenzene, furfuryl alcohol, and 4-vinyl-2-methoxyphenol were detected as metabolites in bacteria and hexadecanol in wetland plant rhizosphere treated PMDE.
    Bioresource Technology 10/2011; 103(1):78-86. DOI:10.1016/j.biortech.2011.09.132 · 4.49 Impact Factor
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    • "Wastewater treatment by conventional biological treatment leads to the large reduction in the organic load but colorants are scarcely degraded. The removal and degradation of the color contributing compounds like melanoidins, caramel, and phenolics and their metabolic products is very essential for the safe disposal of the distillery wastewater to the environmental [13]. Many researchers have tried to use biological processes for removing melanoidins from effluent. "
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    ABSTRACT: Distillery effluent retains very dark brown color even after anaerobic treatment due to presence of various water soluble, recalcitrant and coloring compounds mainly melanoidins. In laboratory conditions, melanoidin decolorizing bacteria was isolated and optimized the cultural conditions at various incubation temperatures, pH, carbon sources, nitrogen sources and combined effect of both carbon and nitrogen sources. The optimum decolorization (72.6 ± 0.56%) of melanoidins was achieved at pH 7.5 and temperature 37 °C on 5th day of cultivation. The toxicity evaluation with mung bean (Vigna radiata) revealed that the raw distillery effluent was environmentally highly toxic as compared to biologically treated distillery effluent, which indicated that the effluent after bacterial treatment is environmentally safe. This proves to be novel biological treatment technique for biodegradation and detoxification of melanoidin from distillery effluent using the bacterial strain SAG(5).
    Journal of hazardous materials 08/2011; 193:319-24. DOI:10.1016/j.jhazmat.2011.07.068 · 4.53 Impact Factor
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