Protective effect of fermented sea tangle against ethanol and carbon tetrachloride-induced hepatic damage in Sprague-Dawley rats

Marinebioprocess Co., Ltd., Busan 619-912, Republic of Korea.
Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association (Impact Factor: 2.9). 02/2010; 48(4):1123-8. DOI: 10.1016/j.fct.2010.02.006
Source: PubMed


Sea tangle has long been used as Korean folk remedy to promote material health, and is one of the popular dietary supplement. This study was designed to evaluate the protective effect of fermented sea tangle (FST) against ethanol and carbon tetrachloride (CCl(4))-induced hepatotoxicity in rats. Sprague-Dawley rats were orally treated with FST (25, 250, 2500 mg/kg/day) with administration of ethanol (5 mL/kg) for 13 weeks and the single intraperitoneal (i.p.) dose of 50% CCl(4) (5 mL/kg/day, CCl(4) in olive oil) at 12 week, and repeated i.p. dose of 20% CCl(4) (2 mL/kg/day) for 1 week. Hepatotoxicity was evaluated by measuring the serum levels of glutamic pyruvate transaminase (GPT), gamma glutamyl transpeptidase (gamma-GT) and malondialdehyde (MDA) as well as the tissue levels of antioxidant enzyme such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Ethanol and CCl(4)-induced the rat liver damage, and significantly increased (p<0.05) the GPT, gamma-GT and MDA levels, and decreased the SOD, CAT and GPx levels. However, treatment with FST could decrease serum GPT, gamma-GT, and MDA levels significantly in plasma, and increase the activities of SOD, CAT, and GPx in liver tissues compared with ethanol and CCl(4)-treated group.

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    • " We have produced fermented L . japonica ( FLJ ) using L . brevis BJ20 , and its increased antioxidant capacity was observed with respect to DPPH scavenging , superoxide radical scavenging , and xanthine oxidase inhibition ( Lee et al . , 2010a ) . Additionally , it effectively protected rats against carbon tetrachloride - induced hepatotoxicity ( Lee et al . , 2010b ) . The objective of this study was to investigate the effect of GABA - enriched FLJ on alcohol - induced hepatotoxicity by assaying hepatic enzyme activities and biochemical markers associated with alcohol metabolism . Materials and Methods Preparation of fermented L . japonica ( FLJ ) L . japonica was added to water at a ratio of 1 : "
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    ABSTRACT: The sea tangle, Laminaria japonica has long been used in Korea as a folk remedy to promote health. Gamma-amino butyric acid-enriched (5.56% of dry weight) sea tangle was obtained by fermentation with Lactobacillus brevis BJ-20 (FLJ). A suppressive effect of FLJ on carbon tetrachloride-induced hepatotoxicity has been shown previously. Alcohol administration to Sprague-Dawley rats leads to hepatotoxicity, as demonstrated by heightened levels of hepatic marker enzymes as well as increases in both the number and volume of lipid droplets as fatty liver progresses. However, FLJ attenuated alcohol-induced hepatotoxicity and the accumulation of lipid droplets following ethanol administration. Additionally, FLJ increased the activities and transcript levels of major alcoholmetabolizing enzymes, such as alcohol dehydrogenase and aldehyde dehydrogenase, and reduced blood concentrations of alcohol and acetaldehyde. These data suggest that FLJ protects against alcohol-induced hepatotoxicity and that FLJ could be used as an ingredient in functional foods to ameliorate the effects of excessive alcohol consumption.
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    • "Several reports have reported that, decreased activities of SOD and CAT in various carcinogenic conditions that may be due to the increased compensation of these anti-oxidant enzymes to neutralize the oxidative stress (Ramakrishnan et al., 2006). The anti-oxidant enzymes SOD, CAT, GPx and GR that are activated against cell injury also limit the effects of oxidant molecules on tissues (Lee et al., 2010). "
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