Article

Sex-preselected buffalo (Bubalus bubalis) calves derived from artificial insemination with sexed sperm.

Animal Reproduction Institute, Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, Guangxi University, Nanning, PR China.
Animal reproduction science (impact factor: 1.56). 01/2010; 119(3-4):169-71. DOI:10.1016/j.anireprosci.2010.01.001 pp.169-71
Source: PubMed

ABSTRACT Flow cytometry sorting of X- and Y-chromosome bearing sperm has been emerging as a promising technology to alter the sex ratio in progenies of mammals in the recent years. The objective of this study was to evaluate the efficiency of AI by using the sexed sperm to produce sex-preselected calves in buffalo species. A total of 43 buffalo cows were inseminated with X-sorted sperm, 30 of which were confirmed pregnant 3 mo following AI. In terms of conception rate, significant difference was observed between AI with sexed sperm derived from different bulls (P<0.05), but not between sexed and non-sexed sperm (P>0.05), nor between heifers and parous buffalo cows (P>0.05). A total of 29 sex-preselected calves, 24 females and 5 males, developed to term and were viable on delivery. Results of this study indicate the feasibility of the application of the sexing technology to accelerate the genetic improvement in swamp buffalo.

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Keywords

24 females
 
29 sex-preselected calves
 
43 buffalo cows
 
5 males
 
conception rate
 
different bulls
 
Flow cytometry sorting
 
genetic improvement
 
non-sexed sperm
 
parous buffalo cows
 
pregnant 3 mo
 
promising technology
 
recent years
 
sex ratio
 
sex-preselected calves
 
sexed sperm
 
sexing technology
 
terms
 
X-sorted sperm
 
Y-chromosome bearing sperm