Article

[Effect of neonatal exposure to environmental pollutants on the DNA methylation of rat testis].

Department of Occupational Health, School of Public Health of Fudan University, Shanghai 200032, China.
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 11/2009; 27(11):649-54. pp.649-54
Source: PubMed

ABSTRACT To investigate the effects of neonatal exposure of DNA methylation inhibitor, Cadmium and PCB153 on DNA methylation, apoptosis and spermatogenesis in SD rats.
Neonatal SD rats were randomly divided into 10 groups and received oral administrations of PCB153 (0.025, 0. 250, 2.500 mg/kg), or Cadmium (1, 2, 4 mg/kg), or positive control 5-Aza-CdR (0.025, 0.250 mg/kg), or vehicle control for five days from PND3. Half of the rats were killed 24 h after the last administration. The remains were fed until 12 weeks. Sperm numbers, apoptosis and DNA methylation levels in testis were investigated.
The daily sperm production was significantly decreased in each neonatal exposed group (P < 0.05). Neonatal rats exposed to 5-Aza-CdR and Cadmium reduced the global DNA methylation level, increased apoptosis, while PCB153 exposure did not significantly change DNA methylation and apoptosis.
Neonatal rats exposed to chemicals could reduce spermatogenesis via multiple pathways. Lower DNA methylation and increased neonatal apoptosis were suggested as one of the causes.

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Keywords

10 groups
 
apoptosis
 
chemicals
 
DNA methylation
 
DNA methylation inhibitor
 
DNA methylation levels
 
global DNA methylation level
 
Lower DNA methylation
 
multiple pathways
 
neonatal apoptosis
 
neonatal exposure
 
Neonatal rats
 
Neonatal SD rats
 
oral administrations
 
PCB153 exposure
 
positive control 5-Aza-CdR
 
SD rats
 
Sperm numbers
 
sperm production
 
vehicle control
 

Ke-Yong Li