Article

Mountant-induced loss of Shorr-stained blue sperm tails.

Centre of Reproductive Medicine and Andrology of the University Münster, Germany.
Asian Journal of Andrology (impact factor: 1.52). 03/2010; 12(2):278-80. DOI:10.1038/aja.2009.93
Source: PubMed

ABSTRACT It has been reported that Shorr staining provides additional morphological information on the motility of spermatozoa in semen, by distinguishing between red and blue flagella. With our routine methods (involving mounting slides) we were unable to confirm these observations. The presence of both red- and blue-coloured sperm tails in Shorr-stained semen smears was apparent, however, if slides were unmounted. Only a very weak association between blue flagellar staining and immotility was observed. Stating whether a mountant was used should be reported.

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Keywords

blue flagella
 
blue flagellar staining
 
blue-coloured sperm tails
 
distinguishing
 
immotility
 
motility
 
observations
 
red-
 
Shorr staining
 
Shorr-stained semen smears
 
weak association
 

Barbara Hellenkemper