In vitro Phytochemical Screening and Antibacterial Activity of Organic Leaf Extracts of Spathodea campanulata P. Beauv Against Hospital Isolated Bacterial Strains

Ethnobotanical Leaflets
Source: OAI


Spathodea campanulata P. Beauv is extensively used in Indian traditional and folklore medicines to cure various human ailments. The preliminary phytochemical screening of the leaves revealed the presence of alkaloids, flavonoids, saponins, tannins, steroids and terpenoids. In vitro antibacterial studies of the leaf extracts were carried out on eight medically important bacterial strains using agar disc diffusion method. The antibacterial assay using petroleum ether leaf extracts showed good inhibitory activity against Klebsiella pneumoniae and compared with standard antibiotic Streptomycin.

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    ABSTRACT: Among plant metabolites, phenolics were found to possess a wide spectrum of biological activities. The purpose of this study was to investigate the ethanol extract of the flowers of Spathodea campanulata P. Beauv. (EEFSC) in terms of bioactivity and composition. Special emphasis was made on its phenolic components. The LD 50 of EEFSC (up to 4 g/kg) indicated its safety. The growth inhibitory activity of EEFSC, and its hexane, chloroform, ethyl acetate and n-butanol fractions was evaluated in-vitro against a set of micro-organisms and 2 human tumor cell lines (breast and colon). The chloroform fraction exhibited the highest antibacterial effect and the least IC 50 against both cell lines. The anthelmintic and acute anti-hyperglycemic potentialities of EEFSC were also explored and found noticeably significant. RP-HPLC with UV detection was employed for identification and quantification of the floral phenolics. Six compounds were isolated from the active chloroform and ethyl acetate/n-butanol fractions.


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