Metabolic flexibility and insulin resistance

Pennington Biomedical Research Center, Baton Rouge, Louisiana 70808, USA.
AJP Endocrinology and Metabolism (Impact Factor: 3.79). 08/2008; 295(5). DOI: 10.1152/ajpendo.90558.2008
Source: OAI


Metabolic flexibility is the capacity for the organism to adapt fuel oxidation to fuel availability. The inability to modify fuel oxidation in response to changes in nutrient availability has been implicated in the accumulation of intramyocellular lipid and insulin resistance. The metabolic flexibility assessed by the ability to switch from fat to carbohydrate oxidation is usually impaired during a hyperinsulinemic clamp in insulin-resistant subjects; however, this "metabolic inflexibility" is mostly the consequence of impaired cellular glucose uptake. Indeed, after controlling for insulin-stimulated glucose disposal rate ( amount of glucose available for oxidation), metabolic flexibility is not altered in obesity regardless of the presence of type 2 diabetes. To understand how intramyocellular lipids accumulate and cause insulin resistance, the assessment of metabolic flexibility to high-fat diets is more relevant than metabolic flexibility during a hyperinsulinemic clamp. An impaired capacity to upregulate muscle lipid oxidation in the face of high lipid supply may lead to increased muscle fat accumulation and insulin resistance. Surprisingly, very few studies have investigated the response to high-fat diets. In this review, we discuss the role of glucose disposal rate, adipose tissue lipid storage, and mitochondrial function on metabolic flexibility. Additionally, we emphasize the bias of using the change in respiratory quotient to calculate metabolic flexibility and propose novel approaches to assess metabolic flexibility. On the basis of current evidence, one cannot conclude that impaired metabolic flexibility is responsible for the accumulation of intramyocellular lipid and insulin resistance. We propose to study metabolic flexibility in response to high- fat diets in individuals having contrasting degree of insulin sensitivity and/or mitochondrial characteristics. National Institutes of Health U01-AG-020478 RO1-DK-60412

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    • "Skeletal muscle is a major consumer of blood glucose under insulin-stimulated conditions [2] [3] and therefore plays a key role in maintaining whole-body glucose homeostasis. Obesity-related skeletal muscle insulin resistance is associated with accumulation of intramyocellular triacylglycerols (TAG), altered carbohydrate and lipid metabolism, resulting in diminished metabolic flexibility and impaired energy production [4], which, in turn, is essential for supporting normal contractile function of skeletal muscle. A number of studies employing rodent models of high-fat-diet (HFD)-induced obesity and insulin resistance have shown that, depending on the content and type of dietary fat as well as the duration of feeding, increased availability of lipids in skeletal muscle may have varying consequences for cellular energy metabolism, including upregulation of genes coding for enzymes of mitochondrial β-oxidation [5] [6], increased mitochondrial capacity to oxidize fatty acids (FAs) and stimulation of mitochondrial biogenesis [7] [8] [9], as well as altered mitochondrial morphology and compromised adenosine triphosphate (ATP) production [7] [10] [11]. "
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    ABSTRACT: High-fat diets (HFD) have been shown to interfere with skeletal muscle energy metabolism and cause peripheral insulin resistance. However, understanding of HFD impact on skeletal muscle primary function – i.e., contractile performance, is limited. Male C57BL/6 J mice were fed HFD containing lard (HFL) or palm oil (HFP), or low-fat diet (LFD) for 5 weeks. Fast-twitch (FT) extensor digitorum longus (EDL) and slow-twitch (ST) soleus muscles were characterized with respect to contractile function and selected biochemical features. In FT EDL muscle, a 30-50% increase in fatty acid (FA) content and doubling of long-chain acylcarnitine (AC) (C14-C18) content in response to HFL and HFP feeding was accompanied by increase in protein levels of peroxisome proliferator-activated receptor-γ coactivator-1α, mitochondrial oxidative phosphorylation complexes and acyl-CoA dehydrogenases involved in mitochondrial FA β-oxidation. Peak force of FT EDL twitch and tetanic contractions were unaltered but the relaxation time (RT) of twitch contractions was 30% slower compared to LFD controls. The latter was caused by accumulation of lipid intermediates rather than changes in the expression levels of proteins involved in calcium handling. In ST soleus muscle, no evidence for lipid overload was found in any HFD group. However, particularly in HFP group, the peak force of twitch and tetanic contractions were reduced but RT was faster than LFD controls. The latter was associated with a fast-to-slow shift in troponin T isoform expression. Taken together, these data highlight fiber-type specific sensitivities and phenotypic adaptations to dietary lipid overload that differentially impact fast- versus slow-twitch skeletal muscle contractile function.
    The Journal of Nutritional Biochemistry 10/2014; 26(2). DOI:10.1016/j.jnutbio.2014.09.014 · 3.79 Impact Factor
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    • "The factors activating AMPK in mammary glands of obese dams are unclear at present. Diet-induced obesity has been shown to impair AMPK regulation by leptin, insulin, and sympathetic neural efferent’s in other tissues [56], and aspects of AMPK function appear to be restored with weight loss [57], [58]. However, leptin and free fatty acids, which are elevated in obese individuals [59], [60] and tend to be higher in lactating obese mice (unpublished data), have also been shown to activate AMPK by stimulating its phosphorylation [61], [62]. "
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    ABSTRACT: Maternal metabolic and nutrient trafficking adaptations to lactation differ among lean and obese mice fed a high fat (HF) diet. Obesity is thought to impair milk lipid production, in part, by decreasing trafficking of dietary and de novo synthesized lipids to the mammary gland. Here, we report that de novo lipogenesis regulatory mechanisms are disrupted in mammary glands of lactating HF-fed obese (HF-Ob) mice. HF feeding decreased the total levels of acetyl-CoA carboxylase-1 (ACC), and this effect was exacerbated in obese mice. The relative levels of phosphorylated (inactive) ACC, were elevated in the epithelium, and decreased in the adipose stroma, of mammary tissue from HF-Ob mice compared to those of HF-fed lean (HF-Ln) mice. Mammary gland levels of AMP-activated protein kinase (AMPK), which catalyzes formation of inactive ACC, were also selectively elevated in mammary glands of HF-Ob relative to HF-Ln dams or to low fat fed dams. These responses correlated with evidence of increased lipid retention in mammary adipose, and decreased lipid levels in mammary epithelial cells, of HF-Ob dams. Collectively, our data suggests that maternal obesity impairs milk lipid production, in part, by disrupting the balance of de novo lipid synthesis in the epithelial and adipose stromal compartments of mammary tissue through processes that appear to be related to increased mammary gland AMPK activity, ACC inhibition, and decreased fatty acid synthesis.
    PLoS ONE 05/2014; 9(5):e98066. DOI:10.1371/journal.pone.0098066 · 3.23 Impact Factor
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    • "Skeletal muscle is also the most important organ in balancing the uptake and oxidation of fatty acids (FA) and is a major consumer of O 2 because it accounts for a large share of the total body mass [9]. Fat overload may represent a metabolic challenge for many apparently healthy individuals, who may be unable to appropriately upregulate skeletal muscle lipid oxidation, resulting in intracellular lipid accumulation and the development of insulin resistance [9] [10]. As a result, skeletal muscle studies are particularly relevant to the characterization of whole-body metabolic flexibility [8]. "
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    ABSTRACT: Postprandial lipemia influences the development of atherosclerosis, which itself constitutes a risk factor for the development of cardiovascular diseases. The introduction of bioactive compounds may prevent these deleterious effects. Proanthocyanidins are potent antioxidants that have hypolipidemic properties, while omega-3 polyunsaturated fatty acids (ω3 PUFAs) stimulate fatty acid oxidation and gene expression programs, controlling mitochondrial functions. In this study, we investigated the effects of acute treatment of ω3 PUFAs and proanthocyanidins on the metabolic flexibility and lipid handling profiles in the skeletal muscle and adipose tissue of rats that were raised on diets, high in saturated fatty acids. For this, oil rich in docosahexaenoic (DHA-OR), grape seed proanthocyanidins extract (GSPE), or both substances (GSPE + DHA-OR) were administered with an overload of lard oil to healthy Wistar rats. Our results indicate that the addition of DHA-OR to lard oil increases insulin sensitivity and redirects fatty acids toward skeletal muscle, thereby activating fatty acid oxidation. We also observed an improvement in adipose mitochondrial functionality and uncoupling. In contrast, GSPE lowers lipidemia, prevents muscle reactive oxygen species (ROS) production and damage, furthermore, activates mitochondrial biogenesis and lipogenesis in adipose tissue. The addition of GSPE+DHA-OR to lard resulted in nearly all the effects of DHA-OR and GSPE administered individually, but the combined administration resulted in a more attenuated profile. © 2013 BioFactors, 2013.
    BioFactors 02/2014; 40(1). DOI:10.1002/biof.1129 · 4.59 Impact Factor
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