Peliosis Hepatis in Cats Is Not Associated With Bartonella henselae Infections

Department of Veterinary Pathology, Freie Universitaet Berlin, Robert-von-Ostertag-Strasse 15, Berlin, Germany.
Veterinary Pathology (Impact Factor: 2.04). 01/2010; 47(1):163-6. DOI: 10.1177/0300985809354347
Source: PubMed

ABSTRACT Peliosis hepatis is a vasculoproliferative disorder of the liver with infectious and noninfectious causes. In humans and dogs, Bartonella henselae has been linked to peliosis hepatis. Although domestic cats are the natural reservoir of B. henselae and although peliosis hepatis is common in this species, an association between this condition and infection with B. henselae has never been investigated in cats. In this study, 26 cases of peliosis hepatis in cats were tested for B. henselae infection by nested polymerase chain reaction and immunohistochemistry. The authors failed to detect B. henselae nucleic acid or antigen in any of the affected liver specimens. These findings suggest that, unlike in humans and dogs, peliosis hepatis in cats may not be significantly associated with a B. henselae infection.

  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Bartonella henselae (Bh) is an emerging zoonotic pathogen that has been associated with a variety of human diseases, including bacillary angiomatosis that is characterized by vasoproliferative tumor-like lesions on the skin of some immunosuppressed individuals. The study of Bh pathogenesis has been limited to in vitro cell culture systems due to the lack of an animal model. Therefore, we wanted to investigate whether the zebrafish embryo could be used to model human infection with Bh. Our data showed that Tg(fli1:egfp)(y1) zebrafish embryos supported a sustained Bh infection for 7 days with >10-fold bacterial replication when inoculated in the yolk sac. We showed that Bh recruited phagocytes to the site of infection in the Tg(mpx:GFP)uwm1 embryos. Infected embryos showed evidence of a Bh-induced angiogenic phenotype and an increase in the expression of genes encoding pro-inflammatory factors and pro-angiogenic markers. However, infection of zebrafish embryos with a deletion mutant in the major adhesin (BadA) resulted in little or no bacterial replication and a diminished host response, providing the first evidence that BadA is critical for in vivo infection. Thus, the zebrafish embryo provides the first practical model of Bh infection that will facilitate efforts to identify virulence factors and define molecular mechanisms of Bh pathogenesis.
    Zebrafish 07/2014; 11(5). DOI:10.1089/zeb.2014.1001 · 1.77 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A novel biosorbent was developed by coating chitosan, a naturally and abundantly available biopolymer, on to activated alumina based on oil shale ash via crosslinking. The adsorbent was characterized by various techniques, such as Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric-differential thermal analysis, and X-ray photoelectron spectroscope. Batch isothermal equilibrium adsorption experiments were condcted to evaluate the adsorbent for the removal of Cu(II) from wastewater. The effect of pH and agitation time on the adsorption capacity was also investigated, indicating that the optimum pH was 6.0. The equilibrium adsorption data were correlated with Langmuir and Freundlich models. The maximum monolayer adsorption capacity of chitosan coated alumina sorbent as obtained from Langmuir adsorption isotherm was found to be 315.46 mg·g−1 for Cu(II). The adsorbent loaded with Cu(II) was readily regenerated using 0.1 mol·L−1 sodium hydroxide solution. All these indicated that chitosan coated alumina adsorbent not only have high adsorption activity, but also had good stability in the wastewater treatment process.
    Chinese Journal of Chemical Engineering 08/2012; 20(4):641–648. DOI:10.1016/S1004-9541(11)60229-2 · 0.87 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Peliosis hepatis (PH) is a rare condition characterized by the presence of blood-filled cavities within the liver. The etiology of PH remains unknown, but it has been reported to be associated with infections or malignancies. However, the cause of PH is not clear in 20-50% of patients. The current study presents the case of a 19-year-old male who presented with right upper quadrant pain that had lasted for three days. The patient was a student with no previous medical history. Contrast-enhanced computer tomography (CT) and ultrasonography showed a neoplasm in the right liver and a diagnosis of primary liver cancer was made due to the manifestation of the disease and the results of physical tests. The individual was treated successfully with an irregular right hemihepatectomy and was in good health at 6-months post-surgery. A tissue specimen was obtained and determined to be PH by pathological examination and immunohistochemistry analysis. Consequently, a diagnosis of PH must be considered in cases like this.
    Oncology letters 10/2013; 6(4):960-962. DOI:10.3892/ol.2013.1479 · 0.99 Impact Factor