Article

Cytotoxic effects of ultra-diluted remedies on breast cancer cells

Integrative Medicine Program-Unit 145, Department of Molecular Pathology, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030-4009, USA.
International Journal of Oncology (Impact Factor: 3.03). 02/2010; 36(2):395-403. DOI: 10.3892/ijo_00000512
Source: PubMed

ABSTRACT The use of ultra-diluted natural products in the management of disease and treatment of cancer has generated a lot of interest and controversy. We conducted an in vitro study to determine if products prescribed by a clinic in India have any effect on breast cancer cell lines. We studied four ultra-diluted remedies (Carcinosin, Phytolacca, Conium and Thuja) against two human breast adenocarcinoma cell lines (MCF-7 and MDA-MB-231) and a cell line derived from immortalized normal human mammary epithelial cells (HMLE). The remedies exerted preferential cytotoxic effects against the two breast cancer cell lines, causing cell cycle delay/arrest and apoptosis. These effects were accompanied by altered expression of the cell cycle regulatory proteins, including downregulation of phosphorylated Rb and upregulation of the CDK inhibitor p27, which were likely responsible for the cell cycle delay/arrest as well as induction of the apoptotic cascade that manifested in the activation of caspase 7 and cleavage of PARP in the treated cells. The findings demonstrate biological activity of these natural products when presented at ultra-diluted doses. Further in-depth studies with additional cell lines and animal models are warranted to explore the clinical applicability of these agents.

0 Followers
 · 
167 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This study concerns the biological effects and physico-chemical variations of extremely diluted solutions (EDS) as a function of aging-time. The biological efficacy of As2O3 at the 45th decimal dilution/succussion (As 45×) was tested in a wheat germination model. Ten trials were carried out from 0 t... [more] This study concerns the biological effects and physico-chemical variations of extremely diluted solutions (EDS) as a function of aging-time. The biological efficacy of As2O3 at the 45th decimal dilution/succussion (As 45×) was tested in a wheat germination model. Ten trials were carried out from 0 to 12 months after treatment preparation, using wheat seeds (Triticum aestivum L.) of the Pandas variety. Seeds were pre-treated by poisoning with 0.1% As2O3 solution to reduce germination, to allow a better evaluation of treatment effects. The outcome variable was the number of non-germinated seeds after 96 h. The As 45× treatment aged for less than three months did not show a significant effect on wheat germination, whereas when aged for longer (3–12 months) the effect became significant. Concerning the physico-chemical characteristics, specific conductivity of As 45×aged from 0 to 12 months was measured, using nine samples for each date. The results showed a clear increasing time trend of specific conductivity, more evident when considering the last three measurements, which correspond to more than six months of aging. The physico-chemical behavior of EDS strongly supports the significant biological effects observed in a wheat model. Frontiers in Life Science. 04/2012; 4(impact factor 1,786). This study concerns the biological effects and physico-chemical variations of extremely diluted solutions (EDS) as a function of aging-time. The biological efficacy of As2O3 at the 45th decimal dilution/succussion (As 45×) was tested in a wheat germination model. Ten trials were carried out from 0 t... [more] This study concerns the biological effects and physico-chemical variations of extremely diluted solutions (EDS) as a function of aging-time. The biological efficacy of As2O3 at the 45th decimal dilution/succussion (As 45×) was tested in a wheat germination model. Ten trials were carried out from 0 to 12 months after treatment preparation, using wheat seeds (Triticum aestivum L.) of the Pandas variety. Seeds were pre-treated by poisoning with 0.1% As2O3 solution to reduce germination, to allow a better evaluation of treatment effects. The outcome variable was the number of non-germinated seeds after 96 h. The As 45× treatment aged for less than three months did not show a significant effect on wheat germination, whereas when aged for longer (3–12 months) the effect became significant. Concerning the physico-chemical characteristics, specific conductivity of As 45×aged from 0 to 12 months was measured, using nine samples for each date. The results showed a clear increasing time trend of specific conductivity, more evident when considering the last three measurements, which correspond to more than six months of aging. The physico-chemical behavior of EDS strongly supports the significant biological effects observed in a wheat model.
    Frontiers in Life Science 04/2012; DOI:10.1080/21553769.2011.638986 · 0.17 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Nelle Mèditationes Cartèsiennes, Husserl afferma "Esperienza è coscienza originale; infatti nel caso dell'esperienza di un uomo in generale noi diciamo che l'altro ci sta innanzi, presente in carne ed ossa. D'altro lato questo tipo di presenza non ci impedisce di ammettere immediata... [more] Nelle Mèditationes Cartèsiennes, Husserl afferma "Esperienza è coscienza originale; infatti nel caso dell'esperienza di un uomo in generale noi diciamo che l'altro ci sta innanzi, presente in carne ed ossa. D'altro lato questo tipo di presenza non ci impedisce di ammettere immediatamente che veramente qui dinanzi a noi non è l'altro io stesso, con i suoi momenti coscienziali e i suoi fenomeni stessi (ossia quel che per lui è fenomeno) e nulla di ciò che appartiene alla sua stessa essenza propria perviene a datità originaria...." L'impossibilità di cogliere l'altro originaliter fa si che si inverte il concetto espresso dal poeta metafisico inglese John Donne:"Nessun uomo è un'isola" nel concetto "ogni uomo è un'isola"
    Isola Isole Insulae, 03/2012: pages 233-235; Johan & Levi., ISBN: 978-88-6010-070-2
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The effects of Arnica montana 6cH on the individual modulation of acute inflammation kinetics in rats were evaluated. Adult male Wistar rats were inoculated with 1% carrageenan into the footpad and treated with Arnica montana 6cH, dexamethasone (4.0 mg/kg; positive control) or 5% hydroalcoholic solution (negative control), per os, each 15 minutes, between 30 and 180 minutes after the irritant inoculation. Histopathological and immunohistochemistry procedures were done in order to get a panel of inflammatory positive cells for CD3 (T lymphocytes), CD45RA (B lymphocytes), CD18 (beta 2 integrin), CD163 (ED2 protein), CD54 (ICAM-1), and MAC 387 (monocytes and macrophages). The statistical treatment of data included a posteriori classification of animals from each group (N = 20) in two subgroups presenting spontaneous precocious or late oedema. Animals that presented precocious oedema were less responsible to Arnica montana 6cH in relation to hemodynamic changes. Instead, rats that exhibited late oedema presented less intense oedema (P = .01), lower percentage of mast cell degranulation (P = .0001), and increase in lymphatic vessels diameter (P = .05). The data suggest an individually qualitative adjustment of inflammatory vascular events by Arnica montana 6cH.
    Evidence-based Complementary and Alternative Medicine 01/2011; 2011:917541. DOI:10.1155/2011/917541 · 1.88 Impact Factor