Article

Rapid discrimination and classification of the Lactobacillus plantarum group based on a partial dnaK sequence and DNA fingerprinting techniques.

Bioresource Collection and Research Center, Food Industry Research and Development Institute, P O Box 246, Hsinchu 30099, Taiwan, ROC.
Antonie van Leeuwenhoek (impact factor: 2.09). 03/2010; 97(3):289-96. DOI:10.1007/s10482-009-9409-5 pp.289-96
Source: PubMed

ABSTRACT The Lactobacillus plantarum group comprises five very closely related species. Some species of this group are considered to be probiotic and widely applied in the food industry. In this study, we compared the use of two different molecular markers, the 16S rRNA and dnaK gene, for discriminating phylogenetic relationships amongst L. plantarum strains using sequencing and DNA fingerprinting. The average sequence similarity for the dnaK gene (89.2%) among five type strains was significantly less than that for the 16S rRNA (99.4%). This result demonstrates that the dnaK gene sequence provided higher resolution than the 16S rRNA and suggests that the dnaK could be used as an additional phylogenetic marker for L. plantarum. Species-specific profiles of the Lactobacillus strains were obtained with RAPD and RFLP methods. Our data indicate that phylogenetic relationships between these strains are easily resolved using sequencing of the dnaK gene or DNA fingerprinting assays.

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  • Article: Rapid identification of Lactobacillus plantarum group using the SNaPshot minisequencing assay.
    Chien-Hsun Huang, Mu-Tzu Chang, Mu-Chiou Huang, Fwu-Ling Lee
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    ABSTRACT: This study used SNaPshot minisequencing for species identification within the Lactobacillus plantarum group. A SNaPshot minisequencing assay using dnaK as a target gene was developed, and five SNP primers were designed by analysing the conserved regions of the dnaK sequences. The specificity of the minisequencing assay was evaluated using 35 strains of L. plantarum group species. The results showed that the SNaPshot minisequencing assay was able to unambiguously and simultaneously discriminate strains belonging to the species L. plantarum subsp. plantarum, L. plantarum subsp. argentoratensis, Lactobacillus paraplantarum, Lactobacillus pentosus and Lactobacillus fabifermentans. In conclusion, a rapid, accurate and cost-effective assay was successfully developed for species identification of the members of the L. plantarum group.
    Systematic and Applied Microbiology 06/2011; 34(8):586-9. · 3.37 Impact Factor
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Keywords

additional phylogenetic marker
 
different molecular markers
 
discriminating phylogenetic relationships
 
DNA fingerprinting
 
DNA fingerprinting assays
 
dnaK
 
dnaK gene
 
dnaK gene sequence
 
food industry
 
Lactobacillus plantarum group
 
Lactobacillus strains
 
RAPD
 
RFLP methods
 
Species-specific profiles
 
type strains