Article

Immunohistochemical expression of estrogens and progesterone receptors in carcinoma ex pleomorphic adenoma-undifferentiated and adenocarcinoma types.

Head of Department of Oral Pathology, Faculty of Dentistry, Aleppo University, Aleppo, Syria.
Medicina oral, patologia oral y cirugia bucal (Impact Factor: 1.02). 05/2010; 15(3):e432-6. DOI: 10.4317/medoral.15.e432
Source: PubMed

ABSTRACT Cancer of the salivary gland is one of the common cancers in the head and the neck regions. This type of cancer develops in the minor and the major salivary glands, and it sometimes metastasizes to other organs, particularly the lung. Morphologic mimicry and similarity in the expression of steroid hormone receptors between salivary gland tumours and breast tumours are well-known phenomena and are occasionally debated in the field of surgical pathology. The expression of sex hormone receptors in some tumours suggests a role for these receptors in tumor pathogenesis and therapy. Previous studies of the expression of estrogens and progesterone receptors in salivary gland tumours have reported conflicting results.
Our study aimed to characterize alteration in the immunohistochemical expression of oestrogens receptor and progesterone receptor in the tumour cells of carcinoma arising in pleomorphic adenoma.
27 cases of carcinoma arising in pleomorphic adenoma (undifferentiated and adenocarcinoma types) were examined.
The results showed that 27 (100 %) of 27 cases had negative nuclear staining for either oestrogens or progesterone receptors.
Our data suggest that carcinomas arising in pleomorphic adenoma were not dependent on endocrine function.

0 Bookmarks
 · 
97 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Carcinoma ex pleomorphic adenoma (Ca ex PA) is a carcinoma arising from a primary or recurrent benign pleomorphic adenoma. It often poses a diagnostic challenge to clinicians and pathologists. This study intends to review the literature and highlight the current clinical and molecular perspectives about this entity. The most common clinical presentation of CA ex PA is of a firm mass in the parotid gland. The proportion of adenoma and carcinoma components determines the macroscopic features of this neoplasm. The entity is difficult to diagnose pre-operatively. Pathologic assessment is the gold standard for making the diagnosis. Treatment for Ca ex PA often involves an ablative surgical procedure which may be followed by radiotherapy. Overall, patients with Ca ex PA have a poor prognosis. Accurate diagnosis and aggressive surgical management of patients presenting with Ca ex PA can increase their survival rates. Molecular studies have revealed that the development of Ca ex PA follows a multi-step model of carcinogenesis, with the progressive loss of heterozygosity at chromosomal arms 8q, then 12q and finally 17p. There are specific candidate genes in these regions that are associated with particular stages in the progression of Ca ex PA. In addition, many genes which regulate tumour suppression, cell cycle control, growth factors and cell-cell adhesion play a role in the development and progression of Ca ex PA. It is hopeful that these molecular data can give clues for the diagnosis and management of the disease.
    Head and Neck Pathology 07/2011; 6(1):1-9.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Carcinoma originating within a pre-existing in pleomorphic adenoma is well-known phenomena and is occasionally debated in the field of surgical pathology. The use of the hormone treatment in salivary gland cancers is controversial. The management of patients who show positive reactivity to androgen receptor in prostate carcinomas has guided the researchers to assess the expression of this receptor in a variety of other tumors, including those arising in the salivary glands and particularly in carcinoma ex pleomorphic adenoma. Our study aimed to characterize alteration in the immunohistochemical expression of androgen receptor in the tumor cells of carcinoma arising in pleomorphic adenoma. 20 cases of carcinoma arising in pleomorphic adenoma (undifferentiated and adenocarcinoma types) were examined. The results showed that 10 (50%) of 20 cases had negative nuclear staining, whereas 10 (50%) of 20 cases had positive nuclear staining for androgen receptor. Our data suggest that carcinoma arising in pleomorphic adenoma may be dependent on endocrine function.
    Medicina oral, patologia oral y cirugia bucal 05/2011; 16(3):e330-4. · 1.02 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The purpose of this study was to evaluate the effects of low doses of estrone on the proliferation, differentiation and mineralization of osteoprecursor cells. The effect on cell viability was determined using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, whereas differentiation and mineralization were examined using an alkaline phosphatase activity test and alizarin red S staining, respectively. The protein expression of estrogen receptor-α (ER-α), estrogen receptor-β (ER-β) and osteopontin (OPN) is assosciated with bone formation. Cell cultures grown in the presence of estrone at concentrations of 0.1 and 1 nM demonstrated an increase in relative values in the MTT assay and cells grown in the presence of estrone at the 10 nM concentration demonstrated an increase in mineralization. The results of the western blot analysis indicated that the addition of estrone upregulated ER-α and ER-β expression, but downregulated the expression of OPN. Based on these findings, it was hypothesized that a low dose of estrone produces positive effects on the mineralization of osteoprecursor cells. Moreover, these results also suggested that higher doses of estrone may be required to significantly enhance the differentiation and mineralization.
    Experimental and therapeutic medicine 10/2012; 4(4):681-684. · 0.34 Impact Factor

Full-text (2 Sources)

View
33 Downloads
Available from
May 29, 2014