Nematicidal Activity of Plant Extracts Against the Root-Knot Nematode, Meloidogyne incognita

The Open Natural Products Journal 10/2009; 2(1). DOI: 10.2174/1874848100902010077
Source: OAI


Nematicidal activity of extracts from plants was assayed against Meloidogyne incognita. In laboratory assays extracts from tobacco (Nicotiana tabacum L), clove (Syzygium aromaticum L), betelvine (Piper betle L), and sweet flag (Acorus calamus L) were most effective in killing the nematode, with an EC50 that was 5-10 times lower than the EC50 of the synthetic pesticides chlorpyrifos, carbosulfan and deltamethrin. The shapes of the dead nematodes differed in a characteristic way, and groups of pesticides and plant extracts could clearly be distinguished based on this phenomenon, which may be an indicator for the modes of action of the tested pesticides. In a greenhouse bioassay clove bud and betelvine were tested as mulch. Experiments revealed that the total number of live nematodes on roots of pepper plants treated with mulch of the clove bud was 7% of that of the controls and did not differ significantly from that of plants treated with the recommended synthetic pesticide carbofuran. The application of clove buds as a botanical pesticide for future use against nematodes is highly promising since clove is the 6th major plant grown on Bangka Island, and the market value of clove has decreased sharply over the last years

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    • "The mechanisms of action of plant extracts may include denaturing and degrading of proteins, inhibition of enzymes and interfering with the electron flow in respiratory chain or with ADP phosphorylation (Elyousr et al., 2010; Taye et al., 2012). Extracts from leaves, seeds, flowers, stems, cloves, rhizomes, roots etc. of different plants reduce nematode population of Meloidogyne, Pratylenchus, Rotylenchulus, Helicotylenchus, Tylenchorhynchus, Radopholus, Tylenchulus, Hoplolaimus, Heterodera, Xiphinema etc. (Wiratno et al., 2009; Radhakrishnan, 2010; Taye et al., 2012; Akpheokhai et al., 2012). Botanicals reduce root gall formation, inhibit the hatching of juveniles and kill nematode (Akpheokhai et al., 2012). "

    • "40 mg/mL, respectively, against Meloidogyne spp. (Wiratno et al. 2009). The concentration-and time-dependent study of prodigiosin and copper sulphate revealed that the prodigiosin possessed high nematicidal potential than copper sulphate at very low concentration and in a very short time (Figure 1). "
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    ABSTRACT: Ineffectiveness of available nematicides and the high damage caused by plant-parasitic nematodes result in the urgent need to find some natural remedy for their control. Bioactivity of the pigment extracted from Serratia marcescens was screened for controlling nematodes at their juvenile stage. Test pigment was found effective against juvenile stages of Radopholus similis and Meloidogyne javanica at low concentrations (LC50 values, 83 and 79 μg/mL, respectively) as compared with positive control of copper sulphate (LC50 values, 380 and 280 μg/mL, respectively). The pigment also exhibited inhibition on nematode egg-hatching ability. Characterisation of extracted pigment with TLC, FTIR, HPLC, HPTLC and spectroscopic analysis confirmed the presence of prodigiosin as a bioactive metabolite. Considering the sensory mechanism of pathogen recognition by nematodes, the use of microbial secondary metabolites can be effective for nematode control rather than using whole organism.
    Natural product research 04/2014; 28(17). DOI:10.1080/14786419.2014.904310 · 0.92 Impact Factor
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    • "). Couroupita quianensis() Pentanema indicum(Pavaraj , 2012), Piper betle(, Wiratno , 2009), Hippobromus pauciflorus Berchemia zeyheri(McGaw , 2007), Ocimum sanctum O. basilicum( "
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    ABSTRACT: A total of 2,714 methanol extracts of 1,677 plant species in 178 families were screened for nemastatic activity against Rhabditis sp. by 96-well microplate bioassay. The plant extracts with a concentration of were mixed with aqueous nematode solution containing about 20 Rhabditis sp. and their activity was examined daily for 7 days. Out of 2,714 plant extracts examined in this test, 2,362 (87.0%) showed no negative influence on the nematode activity, while 187 (6.9%) inhibited nematode activity about 50%, 95 (3.5%) inhibited nematode activity over 90%, and 70 (2.6%) rather enhanced nematode activity. Among those showing over 50% nemastatic activity, 25 extracts were randomly selected for further screening with Rhabditis sp. and with juveniles of Meloidogyne incognita. The screening revealed that 11 extracts (44%) were consistently nemastatic. The 282 extracts with nemastatic activity could be of use in future studies on nematicidal plants.
    03/2014; 20(1). DOI:10.5423/RPD.2014.20.1.037
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