In-line coupling headspace liquid-phase microextraction with capillary electrophoresis

Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
Journal of Chromatography A (Impact Factor: 4.17). 02/2010; 1217(8):1203-7. DOI: 10.1016/j.chroma.2009.12.028
Source: PubMed


An analytical technique of in-line coupling headspace liquid-phase microextraction (HS-LPME) with capillary electrophoresis (CE) was proposed to determine volatile analytes. A special cover unit of the sample vial was adopted in the coupling method. To evaluate the proposed method, phenols were used as model analytes. The parameters affecting the extraction efficiency were investigated, including the configuration of acceptor phase, kind and concentration of acceptor solution, extraction temperature and time, salt-out effect, sample volume, etc. The optimal enrichment factors of HS-LPME were obtained with the sample volume of about half of sample vials, which were confirmed by both the theoretical prediction and experimental results. The enrichment factors were obtained from 520 to 1270. The limits of detection (LODs, S/N=3) were in the range from 0.5 to 1 ng/mL each phenol. The recoveries were from 87.2% to 92.7% and the relative standard deviations (RSDs) were lower than 5.7% (n=6). The proposed method was successfully applied to the quantitative analysis of the phenols in tap water, and proved to be a simple, convenient and reliable sample preconcentration and determination method for volatile analytes in water samples.

6 Reads
  • [Show abstract] [Hide abstract]
    ABSTRACT: Capillary electrophoresis (CE) has become one of the most useful tools in separation science because of its high separation efficiency, low cost, versatility, ease of sample preparation and automation. However, some limitations of CE, such as poor concentration sensitivity due to its lower sample loading and shorter optical path length, limits its further applications in separation science. In order to solve this problem, various on-line sample preconcentration techniques such as transient isotachophoresis preconcentration, field-enhanced sample stacking, micelle to solvent stacking, micelle collapse, dynamic pH junction, sweeping, solid phase extraction, single drop microextraction and liquid phase microextraction have been combined with CE. Recent developments, applications and some variants together with different combinations of these techniques integrating in CE are reviewed here and our discussions will be confined to the past three years (2008–2011).
    Central European Journal of Chemistry 06/2012; 10(3). DOI:10.2478/s11532-012-0007-4 · 1.33 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: As a green sample-pretreatment technique, liquid-phase microextraction (LPME) has been used with gas chromatography, liquid chromatography and capillary electrophoresis (CE). The methodologies combining LPME and CE are performed with extremely small amounts of reagents and solvents.In this review, we summarize the methods for combining LPME and CE published since 1996. We emphasize direct coupling of LPME and CE, including in-line and integrative techniques. We also cover LPME as one step of dual-preconcentration methods for CE analysis.
    TrAC Trends in Analytical Chemistry 07/2010; 29(7):629-635. DOI:10.1016/j.trac.2010.02.017 · 6.47 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: An ionic liquid-based single-drop microextraction (IL-SDME) procedure using IL as an extractant on-line coupled to capillary electrophoresis (CE) is proposed. The method is capable of quantifying trace amounts of phenols in environmental water samples. For the SDME of three phenols, a 2.40 nL IL microdrop was exposed for 10 min to the aqueous sample and then was directly injected into the capillary column for analysis. Extraction parameters such as the extraction time, the IL single-drop volume, pH of the sample solution, ionic strength, volume of the sample solution and the extraction temperature were systematically investigated. Detection limits to three phenols were less than 0.05 microg mL(-1), and their calibration curves were all linear (R(2) > or = 0.9994) in the range from 0.05 to 50 microg mL(-1). And enrichment factors for three phenols were 156, 107 and 257 without agitation, respectively. This method was then utilized to analyze two real environmental samples from Yellow River and tap water, obtaining satisfactory results. Compared with the usual SDME for CE, IL-SDME-CE is a simple, low-cost, fast and environmentally friendly preconcentration technique.
    Journal of Chromatography A 08/2010; 1217(33):5434-9. DOI:10.1016/j.chroma.2010.06.059 · 4.17 Impact Factor
Show more