Zhang MF, Zhang ZY, Fu J, Yang YF, Yun JP.. Correlation between expression of p53, p21/WAF1, and MDM2 proteins and their prognostic significance in primary hepatocellular carcinoma. J Transl Med 7: 110

State Key Laboratory of Oncology in Southern China, Cancer Center of Sun Yat-Sen University, Guangzhou, China.
Journal of Translational Medicine (Impact Factor: 3.93). 12/2009; 7(1, article 110):110. DOI: 10.1186/1479-5876-7-110
Source: PubMed


Tumor Protein p53 (p53), cyclin-dependent kinase inhibitor 1A (p21/WAF1), and murine double minute 2 (MDM2) participate in the regulation of cell growth. Altered expression of these gene products has been found in malignant tumors and has been associated with poor prognosis. Our aim was to investigate the expression of the 3 proteins in hepatocellular carcinoma (HCC) and their prognostic significance.
We examined p53, p21/WAF1, and MDM2 expression in 181 pairs of HCC tissues and the adjacent hepatic tissues by performing immunohistochemistry and examined the expression of the 3 proteins in 7 pairs of HCC tissues and the adjacent hepatic tissues by using western blot analysis.
The expression of p53, p21/WAF1, and MDM2 in the HCC tissues was significantly higher than those in the adjacent hepatic tissues (P < 0.05). A statistical correlation was observed between p53 and p21/WAF1 expression in HCC tissues (R = 0.195, P = 0.008). A statistical correlation was observed between expression of p53 and p21/WAF1 (R = 0.380, P = 0.000), p53 and MDM2 (R = 0.299, P = 0.000), p21/WAF1 and MDM2 (R = 0.285, P = 0.000) in 181 liver tissues adjacent to the tumor. Patients with a low pathologic grade HCC (I+II) had a higher tendency to express p53 on tumor cells than the patients with high pathologic grade HCC (III+IV) (P = 0.007). Survival analysis showed that positive p21/WAF1 expression or/and negative MDM2 expression in HCC was a predictor of better survival of patients after tumor resection (P < 0.05).
The proteins p53, p21/WAF1, and MDM2 were overexpressed in all the HCC cases in this study, and p53 and p21/WAF1 overexpression were positively correlated. The expression of p21/WAF1 and MDM2 can be considered as 2 useful indicators for predicting the prognosis of HCC.

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Available from: Chris Zhiyi Zhang, Oct 06, 2015
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    • "It has been reported that MDM2 protein is overexpressed in HCC tissue. In a previous report by MF Zhang's group, MDM2 expression was examined in 181 pairs of HCC tissues and the adjacent hepatic tissues by using immunohistochemistry, through which the group found that MDM2 was overexpressed in all the HCC cases [49]. But in another study, the result of immunohistochemistry showed that MDM2 was expressed in 26% of HCC, and its expression correlated positively with p53 mutations [50]. "
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    • "Deletion of TP53 was negatively associated with survival and with degree of differentiation. Our findings are in consistence with several reports in other populations showing that positive or negative TP53 expression did not correlate with the survival of the patients with HCC [40] [43] [44]. Table 7 Overall period of survival (survival 2) and the clinicopathologic data "
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