Large-area spatially ordered arrays of gold nanoparticles directed by lithographically confined DNA origami. Nat Nanotechnol 5:121-126

Department of Nanoengineering, 9500 Gilman Drive M/C 0448, University of California San Diego, La Jolla, California 92093-0448, USA.
Nature Nanotechnology (Impact Factor: 33.27). 12/2009; 5(2):121-6. DOI: 10.1038/nnano.2009.450
Source: PubMed

ABSTRACT The development of nanoscale electronic and photonic devices will require a combination of the high throughput of lithographic patterning and the high resolution and chemical precision afforded by self-assembly. However, the incorporation of nanomaterials with dimensions of less than 10 nm into functional devices has been hindered by the disparity between their size and the 100 nm feature sizes that can be routinely generated by lithography. Biomolecules offer a bridge between the two size regimes, with sub-10 nm dimensions, synthetic flexibility and a capability for self-recognition. Here, we report the directed assembly of 5-nm gold particles into large-area, spatially ordered, two-dimensional arrays through the site-selective deposition of mesoscopic DNA origami onto lithographically patterned substrates and the precise binding of gold nanocrystals to each DNA structure. We show organization with registry both within an individual DNA template and between components on neighbouring DNA origami, expanding the generality of this method towards many types of patterns and sizes.

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    • "Various approaches have been taken to control the placement of DNA nanostructures on surfaces. Electron-beam and nanoimprint lithography have been used to pattern hydrophilic regions in a hydrophobic matrix (on the substrate surface) to which DNA origami could physisorb [15] [16] [17]. Other strategies rely on non-covalent interactions, such as ionic attractions on carboxyl-functionalized Au surfaces [18], or physisorption on nanopatterned, and chemically modified, graphene films [19]. "
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    ABSTRACT: In order to exploit the outstanding physical properties of one-dimensional (1D) nanostructures such as carbon nanotubes and semiconducting nanowires and nanorods in future technological applications, it will be necessary to organize them on surfaces with precise control over both position and orientation. Here, we use a 1D rigid DNA motif as a model for studying directed assembly at the molecular scale to lithographically patterned nanodot anchors. By matching the inter-nanodot spacing to the length of the DNA nanostructure, we are able to achieve nearly 100% placement yield. By varying the length of single-stranded DNA linkers bound covalently to the nanodots, we are able to study the binding selectivity as a function of the strength of the binding interactions. We analyze the binding in terms of a thermodynamic model which provides insight into the bivalent nature of the binding, a scheme that has general applicability for the controlled assembly of a broad range of functional nanostructures.
    Nano Research 06/2013; 6(6). DOI:10.1007/s12274-013-0318-6 · 6.96 Impact Factor
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    • "The incubation time was 30 minutes in a closed petri dish. After that time the sample was dipped for 5 seconds into a solution of water and ethanol (50:50 v/v), followed by immersion for one hour in a solution of water in ethanol (10:90 v/v) [8]. All steps were done at room temperature. "
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    ABSTRACT: Self-assembled DNA nanostructures promise low-cost ways to create nanoscale shapes. DNA nanostructures can also be used to position particles with nanometer precision. Yet, reliable and low-cost ways of integrating the structures with MEMS technology still have to be developed and innovations are of great interest to the field. We have examined in detail the adherence of DNA origami tiles on silicon oxide surfaces of wafers in dependence on pH-value and magnesium ion concentration. The results of this work will help to pursue new strategies of positioning DNA nanostruc-tures on SiO2. Precise control over the strength of structure-surface adhesion is a prerequisite of relia-ble processes.
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    ABSTRACT: This review discusses information about the use of DNA as a basis for preparing materials with new properties. The unique molecular recognition property of nucleic acids that underlies the synthesis of targeted controllable structures, where DNA functions as an engineering material rather than a genetic-information carrier, is considered. Causes of significant advances in this field are discussed. The new functional potential of novel materials is examined.
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