Photobacterium gaetbulicola sp. nov., a lipolytic bacterium isolated from a tidal flat sediment.
ABSTRACT A Gram-negative, motile, non-spore-forming and lipolytic bacterial strain, designated Gung47(T), was isolated from a tidal flat on the west coast of Korea. Strain Gung47(T) grew optimally at 30 °C and with 2-5 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain Gung47(T) belonged to the genus Photobacterium. Strain Gung47(T) exhibited 98.1 % 16S rRNA gene sequence similarity with Photobacterium rosenbergii LMG 22223(T) and 94.3-96.3 % similarity with other type strains of species of the genus Photobacterium. Strain Gung47(T) exhibited 47 % DNA-DNA relatedness to P. rosenbergii LMG 22223(T). Strain Gung47(T) contained Q-8 as the predominant ubiquinone and C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH, C(16 : 0) and C(18 : 1)ω7c as the major fatty acids. In this study, two closely related type strains, P. rosenbergii LMG 22223(T) and Photobacterium halotolerans LMG 22194(T), were also found to have Q-8 as the predominant ubiquinone. The DNA G+C content of strain Gung47(T) was 50.6 mol%. The differential phenotypic properties together with the phylogenetic and genetic distinctiveness of strain Gung47(T) demonstrated that this strain is distinguishable from recognized Photobacterium species. Therefore, strain Gung47(T) is considered to represent a novel species of the genus Photobacterium, for which the name Photobacterium gaetbulicola sp. nov. is proposed. The type strain is Gung47(T) (=KCTC 22804(T) =CCUG 58399(T)).
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ABSTRACT: Five novel strains of Photobacterium (A-394T, A-373, A-379, A-397 and A-398) were isolated from bleached coral Madracis decactis (scleractinian) in the remote St Peter & St Archipelago (SPSPA), Mid-Atlantic Ridge, Brazil. Healthy M. decactis specimens were also surveyed, but no strains were related to them. The novel isolates formed a distinct lineage based on the 16S rRNA, recA, and rpoA gene sequences analysis. Their closest phylogenetic neighbours were Photobacterium rosenbergii, P. gaetbulicola, and P. lutimaris, sharing 96.6 to 95.8 % 16S rRNA gene sequence similarity. The novel species can be differentiated from the closest neighbours by several phenotypic and chemotaxonomic markers. It grows at pH 11, produces tryptophane deaminase, presents the fatty acid C18:0, but lacks C16:0 iso. The whole cell protein profile, based in MALDI-TOF MS, distinguished the strains of the novel species among each other and from the closest neighbors. In addition, we are releasing the whole genome sequence of the type strain. The name Photobacterium sanctipauli sp. nov. is proposed for this taxon. The G+C content of the type strain A-394T (=LMG 27910T = CAIM 1892T) is 48.2 mol%.05/2014; DOI:10.7717/peerj.427
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ABSTRACT: A facultative anaerobe, alkalitolerant, gram-negative marine bacterium strain LBS5(T), was isolated from eggs carried on the pleopods of female spiny lobster (Panulirus penicillatus) in Andaman Sea from a depth of 3.5 m. Heterotrophic growth was observed at 15-38 °C and pH 5.5-11. Optimum growth occurred at 28 °C and pH 7.5. It can grow in the presence of 0.5-7 % NaCl (w/v), and the optimal NaCl required for growth was 2-4 %. 16S rRNA gene sequence analysis revealed the strain LBS5(T) belongs to the genus Photobacterium and showed 99.6 % similarity with P. aquae AE6(T), 98.2 % with P. aphoticum M46(T), 97 % with P. rosenbergii CC1(T), 96.9 % with P. lutimaris DF-42(T), and 96.6 % with P. halotolerans MACL01(T). The DNA-DNA similarities between strains LBS5(T) with other closely related strains were well below 70 %. The DNA G + C content was 50.52 (±0.9) mol%. The major fatty acids were C16:1w7c/w6c, C18:1w6c/w7c, C16:0, C15:0 iso, C16:0 10-methyl/17:1 iso w9c, C17:0 iso. Polar lipids included a phosphatidylglycerol, a diphosphatidylglycerol, a phosphatidylethanolamine, and one unidentified lipid. Based on the polyphasic evidences, strain LBS5(T) represents a novel species of the genus Photobacterium for which Photobacterium panuliri sp. nov. is proposed. The type strain is LBS5(T) (=DSM 27646(T) = LMG 27617(T) = JCM 19199(T)).Current Microbiology 06/2014; 69(5). DOI:10.1007/s00284-014-0638-0 · 1.36 Impact Factor
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ABSTRACT: A Gram-staining-negative, heterotrophic, facultatively anaerobic bacterium, designated AE6T, was isolated from a grouper (Epinephelus malabaricas) culture tank in a recirculating mariculture system located in Tianjin, China. Strain AE6T was able to grow at 15-40 oC (optimum, 30-35 oC), at pH 5.5-10.0 (optimum, pH 7.0-7.5) and in the presence of 0.5-7 % (w/v) NaCl (optimum, 2-3 %). It contained Q-8 as the predominant respiratory quinone, phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) as the major polar lipids, and C16:1ω7c/C16:1ω6c (40.4 %), C18:1ω7c (15.5 %) and C16:0 (13.5 %) as the predominant cellular fatty acids. The genome DNA G+C content was 47.1 mol %. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AE6T belonged to the genus Photobacterium (94.2-96.8 % of 16S rRNA gene sequence similarity) and formed a distinct phylogenetic lineage within the genus and exhibited the highest sequence similarities to P. aphoticum CECT 7614T (96.8 %). Multilocus sequence analysis (MLSA) using four loci (gyrB, rpoA, pyrH and recA gene) also revealed that strain AE6T was phylogenetic related to the genus Photobacterium. Based on the phylogenetic, chemotaxonomic and phenotypic evidences, strain AE6T is considered to represent a novel species of the genus Photobacterium, for which the name Photobacterium aquae sp. nov. is proposed. The type strain is AE6T (=CGMCC 1.12159T = JCM 18480T).International Journal of Systematic and Evolutionary Microbiology 10/2013; DOI:10.1099/ijs.0.055020-0 · 2.80 Impact Factor