Photobacterium gaetbulicola sp. nov., a lipolytic bacterium isolated from a tidal flat sediment

Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan, Republic of Korea.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 12/2009; 60(Pt 11):2587-91. DOI: 10.1099/ijs.0.016923-0
Source: PubMed


A Gram-negative, motile, non-spore-forming and lipolytic bacterial strain, designated Gung47(T), was isolated from a tidal flat on the west coast of Korea. Strain Gung47(T) grew optimally at 30 °C and with 2-5 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain Gung47(T) belonged to the genus Photobacterium. Strain Gung47(T) exhibited 98.1 % 16S rRNA gene sequence similarity with Photobacterium rosenbergii LMG 22223(T) and 94.3-96.3 % similarity with other type strains of species of the genus Photobacterium. Strain Gung47(T) exhibited 47 % DNA-DNA relatedness to P. rosenbergii LMG 22223(T). Strain Gung47(T) contained Q-8 as the predominant ubiquinone and C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH, C(16 : 0) and C(18 : 1)ω7c as the major fatty acids. In this study, two closely related type strains, P. rosenbergii LMG 22223(T) and Photobacterium halotolerans LMG 22194(T), were also found to have Q-8 as the predominant ubiquinone. The DNA G+C content of strain Gung47(T) was 50.6 mol%. The differential phenotypic properties together with the phylogenetic and genetic distinctiveness of strain Gung47(T) demonstrated that this strain is distinguishable from recognized Photobacterium species. Therefore, strain Gung47(T) is considered to represent a novel species of the genus Photobacterium, for which the name Photobacterium gaetbulicola sp. nov. is proposed. The type strain is Gung47(T) (=KCTC 22804(T) =CCUG 58399(T)).

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    • "); 3, P. gaetbulicola Gung 47 T (Kim et al., 2010); 4, P. lutimaris LMG 25278 T (Chimetto et al., 2010; Jung et al., 2007); 5, P. jeanii LMG 25436 T (Chimetto et al., 2010; Srinivas et al., 2013); 6, P. leiognathi LMG 4228 T (Baumann & Baumann, 1984; Chimetto et al., 2010; Nogi, Masui & Kato, 1998; Yoshizawa et al., 2009). +, Positive; −, negative; w, weak; v, variable; nd, no data available. "
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    ABSTRACT: Five novel strains of Photobacterium (A-394T, A-373, A-379, A-397 and A-398) were isolated from bleached coral Madracis decactis (scleractinian) in the remote St Peter & St Archipelago (SPSPA), Mid-Atlantic Ridge, Brazil. Healthy M. decactis specimens were also surveyed, but no strains were related to them. The novel isolates formed a distinct lineage based on the 16S rRNA, recA, and rpoA gene sequences analysis. Their closest phylogenetic neighbours were Photobacterium rosenbergii, P. gaetbulicola, and P. lutimaris, sharing 96.6 to 95.8 % 16S rRNA gene sequence similarity. The novel species can be differentiated from the closest neighbours by several phenotypic and chemotaxonomic markers. It grows at pH 11, produces tryptophane deaminase, presents the fatty acid C18:0, but lacks C16:0 iso. The whole cell protein profile, based in MALDI-TOF MS, distinguished the strains of the novel species among each other and from the closest neighbors. In addition, we are releasing the whole genome sequence of the type strain. The name Photobacterium sanctipauli sp. nov. is proposed for this taxon. The G+C content of the type strain A-394T (=LMG 27910T = CAIM 1892T) is 48.2 mol%.
    PeerJ 05/2014; DOI:10.7717/peerj.427 · 2.11 Impact Factor
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    • "The genus Photobacterium, which was first reported by Beijerinck (1889), belonging to the family Vibrionaceae of the class Gammaproteobacteria comprises a group of Gramstain-negative , facultatively aerobic, rod-shaped bacteria that require NaCl for growth. At the time of writing, the genus Photobacterium is composed of the following 23 species and 2 subspecies: Photobacterium aphoticum (Lucena et al., 2011), P. swingsii (Gomez-Gil et al., 2011), P. gaetbulicola (Kim et al., 2010), P. jeanii (Chimetto et al., 2010), P. aquimaris (Yoshizawa et al., 2009), P. kishitanii (Ast et al., 2007), P. lutimaris (Jung et al., 2007), P. ganghwense (Park et al., 2006), P. halotolerans (Rivas et al., 2006), P. lipolyticum (Yoon et al., 2005), P. rosenbergii (Thompson et al., 2005), P. frigidiphilum (Seo et al., 2005a), P. aplysiae (Seo et al., 2005b), P. indicum (Xie & Yokota, 2004), P. iliopiscarium (Urakawa et al., 1999), P. profundum (Nogi et al., 1998), P. damselae subsp. piscicida and P. damselae subsp. "
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    ABSTRACT: A Gram-staining-negative, motile, weakly halophilic and facultatively aerobic bacterium, designated strain YA11T, was isolated from tidal flat sediment at Yeongam bay, South Korea. Strain YA11T grew at 10-30 °C (optimum, 20 °C), at pH 6.0-10.0 (optimum, pH 6.5-7.5) and in the presence of 1-6 % (w/v) NaCl (optimum, 2-3 %). The major cellular fatty acids of the strain were summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c) and C16:0. The DNA G+C content of the genomic DNA was 44.2 mol%. Strain YA11T contained Q-8 as the sole respiratory quinone. Phylogenetic tree based on 16S rRNA gene sequences showed that strain YA11Tformed a distinct phyletic lineage within the genus Photobacterium and the 16S rRNA gene sequence similarities between strain YA11T and the type strains of the genus Photobacterium ranged between 94.0 and 96.4 %. Based on the phenotypic, chemotaxonomic and molecular properties, strain YA11T represents a novel species of the genus Photobacterium, for which the name Photobacterium aestuarii sp. nov. is proposed, with strain YA11T(=KACC 16912T=JCM 18592T) as the type strain.
    International Journal of Systematic and Evolutionary Microbiology 10/2013; 64(Pt 2). DOI:10.1099/ijs.0.056861-0 · 2.51 Impact Factor
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    • "The species P. damselae and P. leiognathi show an unstable association with the P. phosphoreum group. During the writing of this manuscript, two new species of the genus Photobacterium, P. gaetbulicola (Kim et al., 2010) and P. jeanii (Chimetto et al., 2010), were described and as such were included in the analyses. Strain M46 T , isolated from coastal seawater of the Western Mediterranean Sea, was characterized and found to be a new member of the genus Photobacterium and grouped with the third intrageneric cluster, showing the highest 16S rRNA sequence similarity to P. rosenbergii (96.8 %). "
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    ABSTRACT: A facultatively anaerobic marine gammaproteobacterium, designated strain M46(T), was isolated from Mediterranean seawater at Malvarrosa beach, Valencia, Spain. The strain was characterized by using a polyphasic approach and was found to be situated within the genus Photobacterium in the family Vibrionaceae. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46(T) was closely related to P. rosenbergii CECT 7644(T), P. halotolerans CECT 5860(T) and P. ganghwense CECT 7641(T), showing sequence similarities of 96.8, 96.4 and 96.2 %, respectively. According to the results of phylogenetic analyses based on recA and gyrB gene sequences, the most closely related taxon was P. ganghwense CECT 7641(T) with 87.4 and 85.0 % sequence similarity, respectively. Regardless of the gene used in phylogenetic analysis, strain M46(T) always formed a separate and stable clade containing these three species of the genus Photobacterium. Strain M46(T) was not luminescent and produced a diffusible brown pigment. It required NaCl to grow, reduced nitrate to nitrite and oxidized a small number of substrates in Biolog GN plates. Strain M46(T) was positive for arginine dihydrolase (ADH), β-galactosidase, aesculin hydrolysis and DNase activity. In API ZYM tests, the novel strain was positive for alkaline phosphatase, leucine arylamidase and acidic phosphatase activities. The major cellular fatty acids were unsaturated C(18) and C(16), as in other members of the genus Photobacterium, but their relative amounts and the presence or absence of other fatty acids differentiated strain M46(T) from its closest relatives. Based on the results of this polyphasic taxonomic study, strain M46(T) represents a novel species of the genus Photobacterium, for which the name Photobacterium aphoticum is proposed. The type strain is M46(T) ( = CECT 7614(T)  = KCTC 23057(T)).
    International Journal of Systematic and Evolutionary Microbiology 07/2011; 61(Pt 7):1579-84. DOI:10.1099/ijs.0.025171-0 · 2.51 Impact Factor
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