Identification of an Arabidopsis Nodulin-related protein in heat stress

Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, 650223, People's Republic of China.
Moleculer Cells (Impact Factor: 2.09). 12/2009; 29(1):77-84. DOI: 10.1007/s10059-010-0005-3
Source: PubMed


We identified a Nodulin-related protein 1 (NRP1) encoded by At2g03440, which was previously reported to be RPS2 interacting protein in yeast-two-hybrid assay. Northern blotting showed that AtNRP1 expression was suppressed by heat stress (42 degrees C) and induced by low temperature (4 degrees C) treatment. Strong GUS staining was observed in the sites of meristematic tissues of pAtNRP1:: GUS transgenic plants, such as shoot apex and root tips, young leaf veins, stamens and stigmas of flowers, and abscission layers of young siliques. To study AtNRP1 biological functions, we have characterized both loss-of-function T-DNA insertion and transgenic overexpression plants for AtNRP1 in Arabidopsis. The T-DNA insertion mutants displayed no obvious difference as compared to wild-type Arabidopsis under heat stress, but the significant enhanced susceptibility to heat stress was revealed in two independent AtNRP1-overexpressing transgenic lines. Further study found that the decreased thermotolerance in AtNRP1-overexpressing lines accompanied significantly decreased accumulation of ABA after heat treatment, which was probably due to AtNRP1 playing a role in negative-feedback regulation of the ABA synthesis pathway. These results support the viewpoint that the application of ABA inhibits nodulation and nodulin-related gene expression and threaten adverse ambient temperature can impact the nodulin-related gene expression.

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    • "A gene encoding nodulinrelated protein was also found in the CR locus. There is some evidence that a nodulin-related gene was induced and provided a protective defense response when Arabidopsis roots were colonized by several strains of Pseudomonas thivervalensis (Cartieaux et al. 2003), and that this gene plays a role in negative feedback regulation of the ABA-synthesis pathway (Fu et al. 2010). An increase in ABA was observed in Brassica roots during development of clubroot disease (Devos et al. 2005), and was thought to be a signal for protecting seriously clubbed roots from drought (Siemens et al. 2006). "
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    ABSTRACT: The use of clubroot resistance (CR) genes is an effective and economical approach for controlling Plasmodiophora brassicae, the causal agent of clubroot disease in Chinese cabbage (Brassica rapa) and other Brassica crops. In a previous study, we identified and mapped the CRb locus on chromosome A03 of B. rapa in the doubled-haploid (DH) line ‘CR Shinki DH line’ of Chinese cabbage. In this study, CRb, a dominant gene conferring resistance to pathotype 4 of P. brassicae, was finely mapped in combination with bulked segregant analysis and bioinformatics analysis (BIA). Using 1,486 highly susceptible individuals and 2,896 individuals from two separate F2 populations of ‘702-5’ (B. rapa ssp. chinensis) × ‘CR Shinki DH line,’ the CRb locus was narrowed to a region of approximately 0.14 cM between two flanking markers, TCR79 and TCR108. The sequences of seven newly developed markers linked to CRb were landed on bacterial artificial chromosome (BAC) of the reference B. rapa ‘Chiifu-401-42’ by BIA, and a physical map consisting of three BAC clones was constructed. The CRb locus was defined as an interval of approximately 83.5 kb on a BAC clone (KBrB085J21). The target interval contained one Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR–NBS–LRR) gene, one NBS–LRR gene, and several putative regulatory genes in the B. rapa genome. The CRb gene was tightly linked to two other CR genes, CRa and CRb Kato . These results provide useful information for isolation of the CRb gene and tightly linked molecular markers for breeding CR in B. rapa.
    Molecular Breeding 10/2014; 34(3):1173-1183. DOI:10.1007/s11032-014-0108-1 · 2.25 Impact Factor
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    • "Nodulin-related proteins were also reported to play a role in pathogen and heat stress tolerance in A. thaliana (Fu et al. 2010) as well as they may be markers of Rhisobium infection in Vicia faba (Becker et al. 2001). The third gene, a transcript highly similar to dinelactone hydrolase protein family, was predicted to be targeted by conserved miR159c. "
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    ABSTRACT: MicroRNAs (miRNAs) are key players in multiple biological processes; therefore, analysis and characterization of these small regulatory RNAs is a critical step toward a better understanding of animal and plant biology. In apple (Malus domestica), 200 microRNAs are known, which most probably represent only a fraction of miRNAome diversity. As a result, more effort is required to better annotate miRNAs and their functions in this economically important species. We performed deep sequencing of 12 small RNA libraries obtained for fire blight-resistant and fire blight-sensitive trees. In the sequencing results, we identified 116 novel microRNAs and confirmed a majority of previously reported apple miRNAs. We then experimentally verified selected candidates with RT-PCR and stem-loop quantitative PCR (qPCR) and performed differential expression analysis. Finally, we identified and characterized putative targets of all known apple miRNAs. The gene ontology (GO) enrichment analysis suggests prominent roles of miRNAs in response to stresses, including pathogen infection. In this study, we identified 116 new and confirmed the expression of 143 already known miRNAs. Moreover, our data suggests that apple microRNAs might be considered as regulators and markers of fire blight resistance. The analyses we performed allowed us to define four apple miRNAs potentially involved in fire blight resistance in apple trees: mdm-miR169a, mdm-miR160e, mdm-miR167b-g, and mdm-miR168a,b. These miRNAs are known to be involved in response to stresses across other plant species, usually by targeting stress response proteins. The relatively low number of candidates may result from the high variance of biological replicates and the fact that stress response miRNAs are usually induced by the stress factors and frequently expressed at a low level, or not expressed at all, in normal conditions. The results of our studies are freely available in an online database at
    Tree Genetics & Genomes 02/2014; 11(1). DOI:10.1007/s11295-014-0812-3 · 2.45 Impact Factor
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    • "For example, Nodulin-related protein 1 (NRP1) is likely to be involved in the negativefeedback regulation of ABA biosynthesis. Transgenic plants overexpressing NRP1 have reduced ABA accumulation and increased susceptibility to heat stress (Fu et al. 2010). The non-protein amino acid b-aminobutyric acid (BABA) is a xenobiotic compound that can be transported to all the aerial parts of Arabidopsis when watered to the roots (Zimmerli et al. 2008). "
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    ABSTRACT: The growth and development of plants are influenced by the integration of diverse endogenous and environmental signals. Acting as a mediator of extrinsic signals, the stress hormone, abscisic acid (ABA), has been shown to regulate many aspects of plant development in response to unfavourable environmental stresses, allowing the plant to cope and survive in adverse conditions, such as drought, low or high temperature, or high salinity. Here, we summarize recent evidence on the roles of ABA in environmental stress responses in the Arabidopsis root; and on how ABA crosstalks with other phytohormones to modulate root development and growth in Arabidopsis. We also review literature findings showing that, in response to environmental stresses, ABA affects the root system architecture in other plant species, such as rice.
    Plant Cell Reports 04/2013; 32(7). DOI:10.1007/s00299-013-1439-9 · 3.07 Impact Factor
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